Native architecture and acclimation of photosynthetic membranes in a fast-growing cyanobacterium.
Native architecture and acclimation of photosynthetic membranes in a fast-growing cyanobacterium.
复制标题
快速生长的蓝藻中光合膜的原生结构和适应
DOI：
10.1093/plphys/kiac372
复制
发表时间：
2022-10-27
期刊：
影响因子：
7.4
通讯作者：
Liu, Lu-Ning
中科院分区：
文献类型：
--
作者：
Zhao, Long-Sheng;Li, Chun-Yang;Chen, Xiu-Lan;Wang, Qiang;Zhang, Yu-Zhong;Liu, Lu-Ning
天然蛋白质组织的显微镜可视化和快速生长的蓝藻光合机制的适应揭示了其结构异质性和可塑性。光合膜中各种蛋白质复合物的组织、物理关联和生理协调确保了太阳能的高效转换。在这里，我们使用高分辨率原子力显微镜可视化快速生长的蓝细菌细长聚球藻 UTEX 2973 (Syn2973) 类囊体膜内光合复合物的天然结构和相互作用。在 Syn2973 类囊体膜中，观察到光系统 I (PSI) 富集域和晶体光系统 II (PSII) 二聚体阵列，为光合作用电子传输提供了有利的膜环境。适应强光（HL）的类囊体膜容纳了大量的PSI复合物，没有掺入铁应激诱导的蛋白A（IsiA）组装体和IsiA-PSI超复合物的形成。相反，在缺铁 (Fe−) 处理的类囊体膜中，IsiA 蛋白与 PSI 紧密结合，形成具有不同组装结构的 IsiA-PSI 超复合物。此外，I 型 NADH 脱氢酶样复合物 (NDH-1) 在 HL 和 Fe− 条件下上调，并与 PSI 复合物建立密切联系，以促进循环电子传输。我们的研究提供了对环境胁迫下 Syn2973 中光合装置在其天然膜的背景下的结构异质性和可塑性的深入了解。对光合膜组织和适应的深入理解将为揭示有效光捕获和能量转换的分子机制提供一个框架。
Microscopy visualization of the native protein organization and adaptation of the photosynthetic machinery from a fast-growing cyanobacterium reveals its structural heterogeneity and plasticity. Efficient solar energy conversion is ensured by the organization, physical association, and physiological coordination of various protein complexes in photosynthetic membranes. Here, we visualize the native architecture and interactions of photosynthetic complexes within the thylakoid membranes from a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 (Syn2973) using high-resolution atomic force microscopy. In the Syn2973 thylakoid membranes, both photosystem I (PSI)-enriched domains and crystalline photosystem II (PSII) dimer arrays were observed, providing favorable membrane environments for photosynthetic electron transport. The high light (HL)-adapted thylakoid membranes accommodated a large amount of PSI complexes, without the incorporation of iron-stress-induced protein A (IsiA) assemblies and formation of IsiA–PSI supercomplexes. In the iron deficiency (Fe−)-treated thylakoid membranes, in contrast, IsiA proteins densely associated with PSI, forming the IsiA–PSI supercomplexes with varying assembly structures. Moreover, type-I NADH dehydrogenase-like complexes (NDH-1) were upregulated under the HL and Fe− conditions and established close association with PSI complexes to facilitate cyclic electron transport. Our study provides insight into the structural heterogeneity and plasticity of the photosynthetic apparatus in the context of their native membranes in Syn2973 under environmental stress. Advanced understanding of the photosynthetic membrane organization and adaptation will provide a framework for uncovering the molecular mechanisms of efficient light harvesting and energy conversion.
登录
查看更多内容
影响因子：
16.6
作者：
Huokko T;Ni T;Dykes GF;Simpson DM;Brownridge P;Conradi FD;Beynon RJ;Nixon PJ;Mullineaux CW;Zhang P;Liu LN
通讯作者：
Liu LN
影响因子：
7.4
作者：
BURNAP, RL;TROYAN, T;SHERMAN, LA
通讯作者：
SHERMAN, LA
影响因子：
3.2
作者：
Katoh, H;Grossman, AR;Ogawa, T
通讯作者：
Ogawa, T
影响因子：
64.8
作者：
Laughlin, Thomas G.;Bayne, Andrew N.;Davies, Karen M.
通讯作者：
Davies, Karen M.
影响因子：
27.5
作者：
Casella S;Huang F;Mason D;Zhao GY;Johnson GN;Mullineaux CW;Liu LN
通讯作者：
Liu LN