Dissecting the Native Architecture and Dynamics of Cyanobacterial Photosynthetic Machinery.
Dissecting the Native Architecture and Dynamics of Cyanobacterial Photosynthetic Machinery.
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DOI：
10.1016/j.molp.2017.09.019
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发表时间：
2017-11-06
期刊：
影响因子：
27.5
通讯作者：
Liu LN
中科院分区：
文献类型：
--
作者：
Casella S;Huang F;Mason D;Zhao GY;Johnson GN;Mullineaux CW;Liu LN
细胞膜的结构动力学和灵活性在细胞功能中发挥着重要作用，即信号传导、能量转导和生理适应。蓝藻类囊体膜代表了一种可以同时进行含氧光合作用和呼吸作用的模型膜。在这项研究中，我们使用高分辨率原子力、共聚焦和全内反射荧光显微镜，对模型蓝细菌（Synechococcus elongatus PCC 7942）类囊体膜中光合复合物的整体组织和移动性进行了直接可视化。我们在分子水平上可视化了类囊体膜内光系统 I (PSI)、光系统 II (PSII) 和细胞色素 (Cyt) b6f 的自然排列和密集堆积。此外，我们用荧光蛋白分别对 PSI、PSII、Cyt b6f 和 ATP 合酶进行功能标记，揭示了这四种光合复合物的异质分布，并使用活细胞荧光成像确定了它们在拥挤膜环境中的动态特征。我们描述了红光诱导的类囊体膜中光合复合物的聚类定位和可调节扩散，代表了光合装置响应环境变化的重组。了解光合膜的组织和动力学对于合理设计和构建人工光合系统以支持生物能源开发至关重要。对蓝藻类囊体膜的了解也可以扩展到其他细胞膜，例如叶绿体和线粒体膜。类囊体膜是蓝细菌、藻类和高等植物中进行光合作用反应的场所。在这里，我们使用原子力、共焦和全内反射荧光显微镜对蓝藻类囊体膜中光合复合物的天然组织和移动性进行了直接可视化。对蓝藻类囊体膜的了解可以扩展到叶绿体和线粒体膜。
The structural dynamics and flexibility of cell membranes play fundamental roles in the functions of the cells, i.e., signaling, energy transduction, and physiological adaptation. The cyanobacterial thylakoid membrane represents a model membrane that can conduct both oxygenic photosynthesis and respiration simultaneously. In this study, we conducted direct visualization of the global organization and mobility of photosynthetic complexes in thylakoid membranes from a model cyanobacterium, Synechococcus elongatus PCC 7942, using high-resolution atomic force, confocal, and total internal reflection fluorescence microscopy. We visualized the native arrangement and dense packing of photosystem I (PSI), photosystem II (PSII), and cytochrome (Cyt) b6f within thylakoid membranes at the molecular level. Furthermore, we functionally tagged PSI, PSII, Cyt b6f, and ATP synthase individually with fluorescent proteins, and revealed the heterogeneous distribution of these four photosynthetic complexes and determined their dynamic features within the crowding membrane environment using live-cell fluorescence imaging. We characterized red light-induced clustering localization and adjustable diffusion of photosynthetic complexes in thylakoid membranes, representative of the reorganization of photosynthetic apparatus in response to environmental changes. Understanding the organization and dynamics of photosynthetic membranes is essential for rational design and construction of artificial photosynthetic systems to underpin bioenergy development. Knowledge of cyanobacterial thylakoid membranes could also be extended to other cell membranes, such as chloroplast and mitochondrial membranes. The thylakoid membrane is the site for photosynthetic reactions and responses in cyanobacteria, algae and higher plants. Here, we performed direct visualization of the native organization and mobility of photosynthetic complexes in cyanobacterial thylakoid membranes, using atomic force, confocal, and total internal reflection fluorescence microscopy. Knowledge of the cyanobacterial thylakoid membrane could be extended to chloroplast and mitochondrial membranes.
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影响因子：
7.4
作者：
Collins, Aaron M.;Liberton, Michelle;Timlin, Jerilyn A.
通讯作者：
Timlin, Jerilyn A.
影响因子：
3.3
作者：
Agarwal, Rachna;Matros, Andrea;Sainis, Jayashree Krishna
通讯作者：
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DOI：
10.1016/0005-2736(91)90114-n
发表时间：
1991-11-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GUPTE, SS;CHAZOTTE, B;HACKENBROCK, CR
通讯作者：
HACKENBROCK, CR
影响因子：
3.7
作者：
Fraser JM;Tulk SE;Jeans JA;Campbell DA;Bibby TS;Cockshutt AM
通讯作者：
Cockshutt AM
影响因子：
64.8
作者：
Jordan, P;Fromme, P;Krauss, N
通讯作者：
Krauss, N