Early gene expression along the animal-vegetal axis in sea urchin embryoids and grafted embryos.
Early gene expression along the animal-vegetal axis in sea urchin embryoids and grafted embryos.
复制标题
海胆胚状体和嫁接胚胎中沿动植物轴的早期基因表达。
作者:
C. Ghiglione;F. Emily;P. Chang;C. Gache
The HE gene is the earliest strictly zygotic gene activated during sea urchin embryogenesis. It is transiently expressed in a radially symmetrical domain covering the animal-most two-thirds of the blastula. The border of this domain, which is orthogonal to the primordial animal-vegetal axis, is shifted towards the animal pole in Li+-treated embryos. Exogenous micromeres implanted at the animal pole of whole embryos, animal or vegetal halves do not modify the extent and localization of the HE expression domain. In grafted embryos or animal halves, the Li+ effect is not affected by the presence of ectopic micromeres at the animal pole. A Li+-induced shift of the border, similar to that seen in whole embryos, occurs in embryoids developing from animal halves isolated from 8-cell stage embryos or dissected from unfertilised eggs. Therefore, the spatial restriction of the HE gene is not controlled by the inductive cascade emanating from the micromeres and the patterning along the AV-axis revealed by Li+ does not require interactions between cells from the animal and vegetal halves. This suggests that maternal primary patterning in the sea urchin embryo is not limited to a small vegetal center but extends along the entire AV axis.
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DOI:
10.1242/dev.121.5.1497
发表时间:
1995
期刊:
Development (Cambridge, England)
影响因子:
--
作者:
Wikramanayake,AH;Brandhorst,BP;Klein,WH
通讯作者:
Klein,WH
DOI:
10.1016/0168-9525(91)90367-y
发表时间:
1991
期刊:
Trends in genetics : TIG
影响因子:
--
作者:
Cameron,RA;Davidson,EH
通讯作者:
Davidson,EH
DOI:
10.1242/dev.105.3.421
发表时间:
1989
期刊:
Development (Cambridge, England)
影响因子:
--
作者:
Davidson,EH
通讯作者:
Davidson,EH
DOI:
10.1242/dev.108.3.403
发表时间:
1990
期刊:
Development (Cambridge, England)
影响因子:
--
作者:
Livingston,BT;Wilt,FH
通讯作者:
Wilt,FH