Early gene expression along the animal-vegetal axis in sea urchin embryoids and grafted embryos.

Early gene expression along the animal-vegetal axis in sea urchin embryoids and grafted embryos.
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海胆胚状体和嫁接胚胎中沿动植物轴的早期基因表达。

DOI:
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发表时间:
1996
期刊:
影响因子:
4.6
通讯作者:
C. Gache
C. Gache
中科院分区:
生物学2区
文献类型:
--
作者:
C. Ghiglione;F. Emily;P. Chang;C. Gache

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HE基因是海胆胚胎发生过程中最早被激活的严格合子基因。它瞬时表达在一个径向对称的域覆盖动物最三分之二的囊胚。这个域的边界,这是正交的原始动物-植物轴,是向动物极移Li+处理的胚胎。植入整个胚胎、动物或植物半体的动物极的外源微粒不会改变HE表达结构域的范围和定位。在移植的胚胎或动物的一半,Li+效应不受异位微粒在动物极的存在。Li+诱导的边界移动,类似于在整个胚胎中看到的,发生在从8细胞期胚胎分离的动物半体或从未受精卵中解剖的胚状体中。因此,HE基因的空间限制不受来自微粒的诱导级联的控制,并且由Li+揭示的沿AV轴的图案化沿着不需要来自动物和植物半体的细胞之间的相互作用。这表明海胆胚胎中的母体初级图案不限于一个小的植物中心,而是沿着沿着整个AV轴延伸。
The HE gene is the earliest strictly zygotic gene activated during sea urchin embryogenesis. It is transiently expressed in a radially symmetrical domain covering the animal-most two-thirds of the blastula. The border of this domain, which is orthogonal to the primordial animal-vegetal axis, is shifted towards the animal pole in Li+-treated embryos. Exogenous micromeres implanted at the animal pole of whole embryos, animal or vegetal halves do not modify the extent and localization of the HE expression domain. In grafted embryos or animal halves, the Li+ effect is not affected by the presence of ectopic micromeres at the animal pole. A Li+-induced shift of the border, similar to that seen in whole embryos, occurs in embryoids developing from animal halves isolated from 8-cell stage embryos or dissected from unfertilised eggs. Therefore, the spatial restriction of the HE gene is not controlled by the inductive cascade emanating from the micromeres and the patterning along the AV-axis revealed by Li+ does not require interactions between cells from the animal and vegetal halves. This suggests that maternal primary patterning in the sea urchin embryo is not limited to a small vegetal center but extends along the entire AV axis.
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