Microfabricated glucose biosensor for culture well operation.
Microfabricated glucose biosensor for culture well operation.
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用于培养井操作的微型葡萄糖生物传感器。
DOI：
10.1016/j.bios.2012.11.032
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发表时间：
2013
影响因子：
12.6
通讯作者：
J. Hart
中科院分区：
文献类型：
--
作者：
R. Pemberton;T. Cox;R. Tuffin;I. Sage;G. Drago;N. Biddle;J. Griffiths;R. Pittson;G. Johnson;J. Xu;S. Jackson;G. Kenna;R. Luxton;J. Hart
研究了一种含有氧化还原介质酞菁钴（CoPC）和葡萄糖氧化酶（GOx）的水基碳丝网印刷油墨配方在96孔微孔板上制备葡萄糖微生物传感器的适用性：(1)将生物传感器油墨浸涂在铂（Pt）丝电极上，获得满意的安培性能；(2)采用MEMS（微机电系统）微加工技术将油墨沉积在硅衬底上制备的一系列Pt微电极（直径10-500μm）表面：毛细管沉积证明是成功的；采用≥100μm的Pt微盘电极可获得最佳生物传感器性能；(3)采用MEMS工艺在硅96井格式基芯片上制造合适尺寸的金属（Pt）轨迹和衬垫，并将葡萄糖生物传感器墨水丝网印刷到这些衬垫上，以制造葡萄糖微生物传感器。当形成微孔时，使用340μl体积的缓冲液，微生物传感器产生稳态安培响应，在5mM葡萄糖范围内呈线性（n=5个生物传感器的CV=6%）。当使用优化的方案涂覆胶原蛋白以帮助细胞粘附时，生物传感器在培养基中继续表现出令人满意的性能（线性范围至2mM，动态范围至7mM， n=4个生物传感器的CV=5.7%）。最后，这些胶原涂层微生物传感器在5孔96孔格式的微孔中工作，使用5通道多电位器进行测试。观察到葡萄糖引起的电流响应与微孔中的细胞数量之间的关系。这些结果表明，微光刻和丝网印刷技术可以成功地结合在一起，生产出能够监测96个良好格式细胞培养物中葡萄糖代谢的微生物传感器。讨论了这些微生物传感器的潜在应用领域。
A water-based carbon screen-printing ink formulation, containing the redox mediator cobalt phthalocyanine (CoPC) and the enzyme glucose oxidase (GOx), was investigated for its suitability to fabricate glucose microbiosensors in a 96-well microplate format: (1)the biosensor ink was dip-coated onto a platinum (Pt) wire electrode, leading to satisfactory amperometric performance; (2)the ink was deposited onto the surface of a series of Pt microelectrodes (10–500μm diameter) fabricated on a silicon substrate using MEMS (microelectromechanical systems) microfabrication techniques: capillary deposition proved to be successful; a Pt microdisc electrode of ≥100μm was required for optimum biosensor performance; (3)MEMS processing was used to fabricate suitably sized metal (Pt) tracks and pads onto a silicon 96 well format base chip, and the glucose biosensor ink was screen-printed onto these pads to create glucose microbiosensors. When formed into microwells, using a 340μl volume of buffer, the microbiosensors produced steady-state amperometric responses which showed linearity up to 5mM glucose (CV=6% for n=5 biosensors). When coated, using an optimised protocol, with collagen in order to aid cell adhesion, the biosensors continued to show satisfactory performance in culture medium (linear range to 2mM, dynamic range to 7mM, CV=5.7% for n=4 biosensors). Finally, the operation of these collagen-coated microbiosensors, in 5-well 96-well format microwells, was tested using a 5-channel multipotentiostat. A relationship between amperometric response due to glucose, and cell number in the microwells, was observed. These results indicate that microphotolithography and screen-printing techniques can be combined successfully to produce microbiosensors capable of monitoring glucose metabolism in 96 well format cell cultures. The potential application areas for these microbiosensors are discussed.
影响因子：
6.1
作者：
Nie Z;Nijhuis CA;Gong J;Chen X;Kumachev A;Martinez AW;Narovlyansky M;Whitesides GM
通讯作者：
Whitesides GM
影响因子：
7.4
作者：
Chen, XH;Matsumoto, N;Wilson, GS
通讯作者：
Wilson, GS