Microbial production of sabinene--a new terpene-based precursor of advanced biofuel.
Microbial production of sabinene--a new terpene-based precursor of advanced biofuel.
复制标题
微生物生产桧烯——先进生物燃料的新型萜烯前体
DOI：
10.1186/1475-2859-13-20
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发表时间：
2014-02-10
影响因子：
6.4
通讯作者：
Xian M
中科院分区：
文献类型：
--
作者：
Zhang H;Liu Q;Cao Y;Feng X;Zheng Y;Zou H;Liu H;Yang J;Xian M
桧烯是一种在自然界中积累有限的单萜类化合物，正被开发为下一代航空燃料的潜在组分。对先进燃料的需求促使我们开发生物合成路线，从可再生糖中生产桧烯。在这项研究中，桧烯显着生产组装的生物合成途径，使用甲基β 4-磷酸（MEP）或异源甲羟戊酸（MVA）途径结合GPP和桧烯合酶基因在工程大肠杆菌菌株。优化了发酵培养基和工艺条件，提高了桧烯的产量，最高效价可达82.18mg/L。最后，采用优化的培养基和工艺条件进行流加发酵，桧烯的最高浓度为2.65 g/L，平均生产能力为0.018 g h-1 g-1干细胞，甘油转化为桧烯的转化率（g/g）达到3.49%.这是首次报道利用工程菌E.以可再生碳源为原料，因此，确立了桧烯的绿色、可持续生产策略。
Sabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar. In this study, sabinene was significantly produced by assembling a biosynthetic pathway using the methylerythritol 4-phosphate (MEP) or heterologous mevalonate (MVA) pathway combining the GPP and sabinene synthase genes in an engineered Escherichia coli strain. Subsequently, the culture medium and process conditions were optimized to enhance sabinene production with a maximum titer of 82.18 mg/L. Finally, the fed-batch fermentation of sabinene was evaluated using the optimized culture medium and process conditions, which reached a maximum concentration of 2.65 g/L with an average productivity of 0.018 g h-1 g-1 dry cells, and the conversion efficiency of glycerol to sabinene (gram to gram) reached 3.49%. This is the first report of microbial synthesis of sabinene using an engineered E. coli strain with the renewable carbon source as feedstock. Therefore, a green and sustainable production strategy has been established for sabinene.
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影响因子：
18.3
作者：
BROWN, HC;RAMACHANDRAN, PV
通讯作者：
RAMACHANDRAN, PV
影响因子：
2.7
作者：
FUJISAKI, S;NISHINO, T;KATSUKI, H
通讯作者：
KATSUKI, H
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
4.4
作者：
Holtwick, R;Meinhardt, F;Keweloh, H
通讯作者：
Keweloh, H
DOI：
10.1007/s10295-008-0418-6
发表时间：
2008-11-01
影响因子：
3.4
作者：
Ataei, Seyed Ahmad;Vasheghani-Farahani, Ebrahim
通讯作者：
Vasheghani-Farahani, Ebrahim