VOLUME AND ENTHALPY CHANGES UPON PHOTOEXCITATION OF BOVINE RHODOPSIN DERIVED FROM OPTOACOUSTIC STUDIES BY USING AN EQUILIBRIUM BETWEEN BATHORHODOPSIN AND BLUE-SHIFTED INTERMEDIATE
VOLUME AND ENTHALPY CHANGES UPON PHOTOEXCITATION OF BOVINE RHODOPSIN DERIVED FROM OPTOACOUSTIC STUDIES BY USING AN EQUILIBRIUM BETWEEN BATHORHODOPSIN AND BLUE-SHIFTED INTERMEDIATE
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通过使用浴视紫红质和蓝移中间体之间的平衡进行光声研究得出牛视紫红质光激发时的体积和焓变化
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
S. Braslavsky
中科院分区：
文献类型：
--
作者：
T. Gensch;J. Straßburger;W. Gärtner;S. Braslavsky
首次使用包括平衡在内的复杂多步骤模型来分析通过激光诱导光声光谱 (LIOAS) 获得的数据，其中牛视紫红质在洗过的膜中 (T = 5–32°C)。热平衡位于红视紫红质、batho 和蓝移中间体 (BSI) 之间，即 Batho ⇄ BSI。该分析考虑了 LIOAS 在 25°C 下检测到的 14 ns 中间体（具有与溶解视紫红质中 BSI 报告的相似的衰变激活参数）。对于基于简单的 bado BSI lumi 序列的模型和包含 bado ⇄ 的模型，lumirhodopsin (lumi) 的能量含量以及 lumi 和视紫红质之间的结构体积差异基本相同（Elumi. = 80 kJ/mol 和 ΔVlumi–rho = 40 mL/mol）； BSI 平衡。然而，引入平衡后，batho BSI 和 BSI lumi 各个步骤的结构体积变化发生了显着变化。 Hug 等人确定了溶解视紫红质的正向反应 Batho BSI 的速率常数。 (Hug, S.J.; Lewis, W.J.; Einterz, C.M.; Thorgeirsson, T.E.; Kliger, D.S. Biochemistry, 1990, 29, 1475–1485) 使用平衡模型获得的结构体积变化为 ΔVBSI-batho = 79 mL/mol 和 ΔVlumi-BSI = -44 mL/mol。由此产生的 Batho BSI 膨胀比顺序模型更大，并且 ΔVlumi-BSI 现在是收缩而不是膨胀。两种模型均推导出 5 mL/mol 的小膨胀，以形成 Batho。 BSI的估计能量含量明显高于lumi的值。由平衡模型得出的 Batho、BSI 和 Lumi 之间的结构体积变化比来自简单序列模型的结构体积变化与文献中使用线性二色性测量确定的发色团跃迁偶极矩变化的相关性更好。结构体积的变化是根据发色团-蛋白质相互作用的变化来讨论的。
A complex multistep model including an equilibrium was used for the first time to analyze data obtained by laser-induced optoacoustic spectroscopy (LIOAS) with bovine rhodopsin in washed membranes (T = 5–32°C). The thermal equilibrium was between bathorhodopsin, batho, and the blue-shifted intermediate (BSI), i.e., batho ⇄ BSI. This analysis considers a 14-ns intermediate detected by LIOAS at 25°C (with similar decay activation parameters as those reported for BSI in solubilized rhodopsin). The energy content of lumirhodopsin (lumi) and the structural volume difference between lumi and rhodopsin were essentially the same (Elumi. = 80 kJ/mol and ΔVlumi–rho = 40 mL/mol) for both a model based on a simple batho BSI lumi sequence and one including the batho ⇄; BSI equilibrium. However, the structural volume changes for the individual steps batho BSI and BSI lumi were markedly changed by the introduction of the equilibrium. With the rate constant for the forward reaction batho BSI for solubilized rhodopsin determined by Hug et al. (Hug, S.J.; Lewis, W.J.; Einterz, C.M.; Thorgeirsson, T.E.; Kliger, D.S. Biochemistry, 1990, 29, 1475–1485) the structural volume changes obtained with the equilibrium model are ΔVBSI-batho = 79 mL/mol and ΔVlumi-BSI = −44 mL/mol. The resulting batho BSI expansion is larger than with the sequential model, and ΔVlumi-BSI is now a contraction instead of an expansion. A small expansion of 5 mL/mol is derived for the formation of batho with both models. The estimated energy content of BSI is clearly higher than the value of lumi. The structural volume changes between batho, BSI, and lumi derived from the equilibrium model correlate better than those from the simple sequential model with the chromophore transition dipole moment changes determined in the literature using linear dichroism measurements. The structural volume changes are discussed in terms of the variations upon transformation of the chromophore–protein interactions.
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脉冲激光光声学的反卷积分析。
影响因子：
--
作者：
Small,JR
通讯作者：
Small,JR
视紫红质席夫碱抗衡离子突变体早期光解中间体的时间分辨光谱。
DOI：
10.1021/bi962320u
发表时间：
1997
期刊：
Biochemistry.
影响因子：
--
作者：
Jager,S;Lewis,JW;Zvyaga,TA;Szundi,I;Sakmar,TP;Kliger,DS
通讯作者：
Kliger,DS
影响因子：
3.4
作者：
Amnon Albeck;N. Friedman;M. Ottolenghi;M. Sheves;C. M. Einterz;S. Hug;J. W. Lewis;D. Kliger
通讯作者：
Amnon Albeck;N. Friedman;M. Ottolenghi;M. Sheves;C. M. Einterz;S. Hug;J. W. Lewis;D. Kliger
影响因子：
2.9
作者：
HUG, SJ;LEWIS, JW;KLIGER, DS
通讯作者：
KLIGER, DS
影响因子：
56.9
作者：
SCHOENLEIN, RW;PETEANU, LA;SHANK, CV
通讯作者：
SHANK, CV