Glycoproteins and glycosaminoglycans of cultured normal human epidermal keratinocytes.
Glycoproteins and glycosaminoglycans of cultured normal human epidermal keratinocytes.
复制标题
培养的正常人表皮角质形成细胞的糖蛋白和糖胺聚糖。
作者：
K. Brown;E. Parkinson
[3H]葡萄糖胺已被用于标记代谢角化细胞表面的糖缀合物。在十二烷基硫酸钠/聚丙烯酰胺凝胶上鉴定的主要标记带的表观分子量大于25万，15万~ 8万。这些成分大多是胰蛋白酶敏感的，表明标签是蛋白质结合的。经胰蛋白酶释放的糖胺聚糖被鉴定为透明质酸（54%）、硫酸肝素（33%）和硫酸软骨素（13%）。特异性免疫方法（免疫过氧化物酶染色和免疫沉淀）显示角质形成细胞产生纤维连接蛋白。免疫过氧化物酶染色显示角化细胞仅在细胞边缘产生纤维连接蛋白的小“针”；未见大纤维，细胞上或细胞间未见染色。
[3H]glucosamine has been used to label metabolically keratinocyte cell-surface glycoconjugates. The major labelled bands identified on sodium dodecyl sulphate/polyacrylamide gels had apparent molecular weights of greater than 250 000, and 150 000-80 000. Most of these components were trypsin-sensitive, indicating that the label was protein-bound. Some of the labelled components were shown to be proteoglycans and the labelled glycosaminoglycans released from them by trypsin were identified as hyaluronic acid (54%), heparan sulphate (33%) and chondroitin sulphate (13%). Specific immunological methods (immunoperoxidase staining and immunoprecipitation) showed that keratinocytes produced fibronectin. Immunoperoxidase staining showed keratinocytes produce only small 'stitches' of fibronectin at cell edges; no large fibrils were seen nor any staining over or between cells.
一种新的连续单维电泳系统，用于分离和定量单个糖胺聚糖。
影响因子：
2.9
作者：
Schuchman,EH;Desnick,RJ
通讯作者：
Desnick,RJ