Central CRF neurons are not created equal: phenotypic differences in CRF-containing neurons of the rat paraventricular hypothalamus and the bed nucleus of the stria terminalis.
Central CRF neurons are not created equal: phenotypic differences in CRF-containing neurons of the rat paraventricular hypothalamus and the bed nucleus of the stria terminalis.
复制标题
DOI：
10.3389/fnins.2013.00156
复制
发表时间：
2013
影响因子：
4.3
通讯作者：
Rainnie DG
中科院分区：
文献类型：
--
作者：
Dabrowska J;Hazra R;Guo JD;Dewitt S;Rainnie DG
促肾上腺皮质激素释放因子（CRF）在启动许多内分泌、自主神经和行为应激反应中起着关键作用。下丘脑室旁核（PVN）的CRF神经元通过激活应激轴参与调节内分泌功能。然而，CRF也被认为在介导对环境压力的焦虑样反应中起关键作用，并且下丘脑外脑区域（如终纹床核（BNST））中的CRF系统的功能障碍与许多精神障碍（包括焦虑和抑郁）的病因学有关。因此，虽然CRF神经元的PVN和BNST共享一个共同的神经肽表型，他们可能代表两个功能不同的神经元群体。在这里，我们采用双免疫荧光，单细胞RT-PCR，和电生理技术，以进一步研究这个问题，并报告说，CRF神经元的PVN和BNST是根本不同的，PVN CRF神经元是谷氨酸能，而BNST CRF神经元是GABA能。此外，这两个神经元群体可以基于它们的电生理特性、它们的肽神经递质（如催产素和精氨酸-加压素）的共表达以及它们的同源受体来进一步区分。我们的研究结果表明，CRF神经元在PVN和BNST不仅会在他们的反应不同的局部神经递质释放，但也在他们的行动下游靶结构。
Corticotrophin-releasing factor (CRF) plays a key role in initiating many of the endocrine, autonomic, and behavioral responses to stress. CRF-containing neurons of the paraventricular nucleus of the hypothalamus (PVN) are classically involved in regulating endocrine function through activation of the stress axis. However, CRF is also thought to play a critical role in mediating anxiety-like responses to environmental stressors, and dysfunction of the CRF system in extra-hypothalamic brain regions, like the bed nucleus of stria terminalis (BNST), has been linked to the etiology of many psychiatric disorders including anxiety and depression. Thus, although CRF neurons of the PVN and BNST share a common neuropeptide phenotype, they may represent two functionally diverse neuronal populations. Here, we employed dual-immunofluorescence, single-cell RT-PCR, and electrophysiological techniques to further examine this question and report that CRF neurons of the PVN and BNST are fundamentally different such that PVN CRF neurons are glutamatergic, whereas BNST CRF neurons are GABAergic. Moreover, these two neuronal populations can be further distinguished based on their electrophysiological properties, their co-expression of peptide neurotransmitters such as oxytocin and arginine-vasopressin, and their cognate receptors. Our results suggest that CRF neurons in the PVN and the BNST would not only differ in their response to local neurotransmitter release, but also in their action on downstream target structures.
登录
查看更多内容
影响因子：
5.5
作者：
FREUNDMERCIER, MJ;RICHARD, P
通讯作者：
RICHARD, P
影响因子：
3.2
作者：
Arima, H;Aguilera, G
通讯作者：
Aguilera, G
影响因子：
3.2
作者：
Bosch, O. J.;Pfoertsch, J.;Neumann, I. D.
通讯作者：
Neumann, I. D.
影响因子：
10.6
作者：
Dabrowska, Joanna;Hazra, Rimi;Guo, Ji-Dong;Li, ChenChen;DeWitt, Sarah;Xu, Jian;Lombroso, Paul J.;Rainnie, Donald G.
通讯作者：
Rainnie, Donald G.
影响因子：
2.5
作者：
Crane, JW;Buller, KM;Day, TA
通讯作者：
Day, TA