Rapid prototyping of arrayed microfluidic systems in polystyrene for cell-based assays.
Rapid prototyping of arrayed microfluidic systems in polystyrene for cell-based assays.
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DOI：
10.1021/ac102897h
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发表时间：
2011-02-15
影响因子：
7.4
通讯作者：
Beebe, David J.
中科院分区：
文献类型：
--
作者：
Young, Edrnond W. K.;Berthier, Erwin;Guckenberger, David J.;Sackmann, Eric;Lamers, Casey;Meyvantsson, Ivar;Huttenocher, Anna;Beebe, David J.
基于细胞的微流控系统使得能够研究具有改进的微环境时空控制和增加的通量的细胞现象。虽然PDMS已成为微流体研究中最受欢迎的材料，但它具有特定的局限性，阻碍了微流体平台充分发挥其潜力。我们在这里提出了一个完整的过程，从模具设计到压花和粘合，描述了聚苯乙烯（PS）微流体设备的制造与基于PDMS的设备具有类似的成本和时间支出。重点放在创建方法，可以与PDMS制造方法的鲁棒性，复杂性和时间要求方面的竞争。为了实现这一目标，进行了几项改进，以消除现有PS压花方法中的关键瓶颈。首先，传统的光刻技术适用于制造能够抵抗高温和高压的大体积环氧树脂模具。其次，开发了一种方法来在PS层中形成通孔，从而能够在单个设备上创建独立的微流体系统的大阵列，而无需手动创建访问端口。第三，PS层的热键合进行了优化，以实现质量键合的大型阵列的微系统。使用两种不同的基于细胞的应用验证了材料和方法的选择的生物功能，以证明我们的流线型制造工艺的多功能性。
Microfluidic cell-based systems have enabled the study of cellular phenomena with improved spatiotemporal control of the microenvironment and at increased throughput. While PDMS has emerged as the most popular material in microfluidics research, it has specific limitations that prevent microfluidic platforms from achieving their full potential. We present here a complete process, ranging from mold design to embossing and bonding, that describes the fabrication of polystyrene (PS) microfluidic devices with similar cost and time expenditures as PDMS-based devices. Emphasis was placed on creating methods that can compete with PDMS fabrication methods in terms of robustness, complexity and time requirements. To achieve this goal several improvements were made to remove critical bottlenecks in existing PS embossing methods. First, traditional lithography techniques were adapted to fabricate bulk epoxy molds capable of resisting high temperatures and pressures. Second, a method was developed to emboss through-holes in a PS layer, enabling creation of large arrays of independent microfluidic systems on a single device without need to manually create access ports. Third, thermal bonding of PS layers was optimized in order to achieve quality bonding over large arrays of microsystems. The choice of materials and methods were validated for biological function using two different cell-based applications to demonstrate the versatility of our streamlined fabrication process.
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影响因子：
2.7
作者：
Jo, BH;Van Lerberghe, LM;Beebe, DJ
通讯作者：
Beebe, DJ
影响因子：
7.4
作者：
Heo, Yun Seok;Cabrera, Lourdes M.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
5.5
作者：
KNOFF, WF;HOPKINS, IL;TOBOLSKY, AV
通讯作者：
TOBOLSKY, AV
DOI：
10.1126/science.1188302
发表时间：
2010-06-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Huh D;Matthews BD;Mammoto A;Montoya-Zavala M;Hsin HY;Ingber DE
通讯作者：
Ingber DE
影响因子：
56.9
作者：
BEVILACQUA, MP;STENGELIN, S;SEED, B
通讯作者：
SEED, B