Arabidopsis membrane-associated acyl-CoA-binding protein ACBP1 is involved in stem cuticle formation.
Arabidopsis membrane-associated acyl-CoA-binding protein ACBP1 is involved in stem cuticle formation.
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DOI：
10.1093/jxb/eru304
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发表时间：
2014-10
影响因子：
6.9
通讯作者：
Chye ML
中科院分区：
文献类型：
--
作者：
Xue Y;Xiao S;Kim J;Lung SC;Chen L;Tanner JA;Suh MC;Chye ML
重组AtACBP 1与极长链酰基辅酶A酯的结合与AtACBP 1在拟南芥茎角质层代谢中的功能有关。功能丧失突变对茎角质层的组成和结构产生不利影响。拟南芥乙酰辅酶A结合蛋白1（AtACBP 1）是一种膜锚定蛋白，在胚胎发生和非生物胁迫反应中起重要作用，并与长链乙酰辅酶A酯相互作用。在这里，AtACBP 1在茎角质层形成中的功能进行了研究。用AtACBP 1 pro：：GUS构建体转化的转基因拟南芥揭示了β-葡萄糖醛酸酶（GUS）在茎（而非叶）表面上的表达，表明在茎角质层形成中的特定作用。等温滴定量热法结果显示，（His）6-标记的重组AtACBP 1与LC酰基辅酶A酯（18：1-、18：2-和18：3-辅酶A）和极长链（VLC）酰基辅酶A酯（24：0-、25：0-和26：0-辅酶A）相互作用。VLC脂肪酸先前已被证明在蜡生物合成中充当前体。气相色谱（GC）-火焰离子化检测器（FID）和气相色谱-质谱（MS）分析表明，acbp 1突变体表现出减少茎和叶角质蜡和茎角质单体组成相比，野生型（Col-0）。因此，acbp 1突变体在扫描电子显微镜和不规则的茎角质层的茎表面上的蜡晶体显示较少的透射电子显微镜与野生型相比。此外，突变体茎始终表现出下降的角质蜡和角质生物合成基因的表达与野生型相比，和突变体叶片更容易受到感染的坏死型病原体灰葡萄孢。综上所述，这些研究结果表明，AtACBP 1参与拟南芥茎角质层的形成贩运VLC酰基辅酶A。
The binding of recombinant AtACBP1 to very-long-chain acyl-CoA esters is related to AtACBP1 function in Arabidopsis stem cuticle metabolism. Loss-of-function mutation adversely affected stem cuticle composition and structure. The membrane-anchored Arabidopsis thaliana ACYL-COA-BINDING PROTEIN1 (AtACBP1) plays important roles in embryogenesis and abiotic stress responses, and interacts with long-chain (LC) acyl-CoA esters. Here, AtACBP1 function in stem cuticle formation was investigated. Transgenic Arabidopsis transformed with an AtACBP1pro::GUS construct revealed β-glucuronidase (GUS) expression on the stem (but not leaf) surface, suggesting a specific role in stem cuticle formation. Isothermal titration calorimetry results revealed that (His)6-tagged recombinant AtACBP1 interacts with LC acyl-CoA esters (18:1-, 18:2-, and 18:3-CoAs) and very-long-chain (VLC) acyl-CoA esters (24:0-, 25:0-, and 26:0-CoAs). VLC fatty acids have been previously demonstrated to act as precursors in wax biosynthesis. Gas chromatography (GC)–flame ionization detector (FID) and GC–mass spectrometry (MS) analyses revealed that an acbp1 mutant showed a reduction in stem and leaf cuticular wax and stem cutin monomer composition in comparison with the wild type (Col-0). Consequently, the acbp1 mutant showed fewer wax crystals on the stem surface in scanning electron microscopy and an irregular stem cuticle layer in transmission electron microscopy in comparison with the wild type. Also, the mutant stems consistently showed a decline in expression of cuticular wax and cutin biosynthetic genes in comparison with the wild type, and the mutant leaves were more susceptible to infection by the necrotrophic pathogen Botrytis cinerea. Taken together, these findings suggest that AtACBP1 participates in Arabidopsis stem cuticle formation by trafficking VLC acyl-CoAs.
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影响因子：
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作者：
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通讯作者：
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