Complete genome sequence of a Capsicum chlorosis virus in China and the structural variation and evolutionary origin of its S RNA intergenic region

Complete genome sequence of a Capsicum chlorosis virus in China and the structural variation and evolutionary origin of its S RNA intergenic region
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中国辣椒失绿病毒全基因组序列及其S RNA基因间区的结构变异和进化起源

DOI:
10.1007/s00705-017-3448-4
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发表时间:
2017-06
影响因子:
2.7
通讯作者:
Tao Xiao-Rong
Tao Xiao-Rong
中科院分区:
医学4区
文献类型:
--
作者:
Huang Ying;Hong Hao;Zhao Xiao-Hui;Li Jia;Tao Xiao-Rong

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测定了中国辣椒褪绿病毒(CaCV-Hainan)的全基因组序列。CaCV-海南株的基因组由小RNA(S)、中RNA(M)和大RNA(L)组成,分别为3629、4859和8912个核苷酸(nt)。S和M RNA分别含有1348和462 nt的基因间区(IGR)。引人注目的是,CaCV分离株之间的序列比较显示,CaCV-海南的S RNA IGR通过缺失CaCV-Qld-3432的S RNA IGR内的两段25-和325-nt序列而源自CaCV-Qld-3432澳大利亚分离株。此外,CaCV-海南的S RNA IGR插入了两段未知来源的10-和20-nt序列。来自台湾的CaCV-Ph和来自泰国的CaCV-NRA的S RNA IGR也通过缺失218-nt序列而衍生自CaCV-Qld-3432。我们的研究结果为CaCV IGRs的结构变异和进化起源提供了有价值的新见解。
The complete genome sequence of a Capsicum chlorosis virus from China (CaCV-Hainan) was determined. The tripartite genome of CaCV-Hainan consists of small (S), medium (M), and large (L) RNAs of 3629, 4859, and 8912 nucleotides (nt), respectively. The S and M RNAs contain intergenic regions (IGRs) of 1348 and 462 nt, respectively. Strikingly, sequence comparisons among CaCV isolates revealed that the S RNA IGR of CaCV-Hainan derived from the CaCV-Qld-3432 Australia isolate through deletion of two stretches of 25- and 325-nt sequences within the S RNA IGR of CaCV-Qld-3432. Moreover, the S RNA IGR of CaCV-Hainan was inserted with two stretches of 10- and 20-nt sequences of an unknown origin. The S RNA IGR of CaCV-Ph from Taiwan and CaCV-NRA from Thailand also derived from the CaCV-Qld-3432 through deletion of 218-nt sequences. Our findings provide valuable new insight into the structural variations and evolutionary origin of CaCV IGRs.
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