Effects of Morphology vs. Cell-Cell Interactions on Endothelial Cell Stiffness.
Effects of Morphology vs. Cell-Cell Interactions on Endothelial Cell Stiffness.
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DOI：
10.1007/s12195-010-0142-y
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发表时间：
2011-03-01
影响因子：
2.8
通讯作者：
Aranda-Espinoza, Helim
中科院分区：
文献类型：
--
作者：
Stroka, Kimberly M.;Aranda-Espinoza, Helim
动脉粥样硬化形成、伤口愈合、癌细胞转移和免疫细胞迁移等生物学过程依赖于细胞-细胞和细胞-基质粘附之间的微妙平衡。细胞力学已被证明取决于基板因素，如刚度和配体的介绍，而细胞-细胞相互作用对细胞的机械性能的影响很少受到关注。在这里，我们使用原子力显微镜测量活的人脐静脉内皮细胞（HUVEC）的杨氏模量。在改变HUVEC中细胞-细胞接触的程度（单细胞、组和单层）时，我们观察到细胞硬度增加与细胞面积增加相关。此外，我们观察到HUVEC在它们扩散到玻璃基板上时会扩散。当我们削弱细胞-细胞连接时（即，通过低剂量的细胞松弛素B或用VE-钙粘蛋白抗体处理），我们观察到细胞-基质粘附增加，如通过粘着斑尺寸和密度所测量的，并且单层内细胞的硬度接近单细胞的硬度。我们的研究结果表明，虽然形态学可以大致用于预测细胞刚度，细胞-细胞相互作用可能发挥重要作用，在确定组织中的单个细胞的机械性能，通过小心维护细胞张力稳态。
Biological processes such as atherogenesis, wound healing, cancer cell metastasis, and immune cell transmigration rely on a delicate balance between Cell–Cell and cell–substrate adhesion. Cell mechanics have been shown to depend on substrate factors such as stiffness and ligand presentation, while the effects of Cell–Cell interactions on the mechanical properties of cells has received little attention. Here, we use atomic force microscopy to measure the Young’s modulus of live human umbilical vein endothelial cells (HUVECs). In varying the degree of Cell–Cell contact in HUVECs (single cells, groups, and monolayers), we observe that increased cell stiffness correlates with an increase in cell area. Further, we observe that HUVECs stiffen as they spread onto a glass substrate. When we weaken Cell–Cell junctions (i.e., through a low dose of cytochalasin B or treatment with a VE-cadherin antibody), we observe that cell–substrate adhesion increases, as measured by focal adhesion size and density, and the stiffness of cells within the monolayer approaches that of single cells. Our results suggest that while morphology can roughly be used to predict cell stiffness, Cell–Cell interactions may play a significant role in determining the mechanical properties of individual cells in tissues by careful maintenance of cell tension homeostasis.
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影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
影响因子：
8.3
作者：
Blacher, J;Asmar, R;Safar, ME
通讯作者：
Safar, ME
影响因子：
3.7
作者：
Chouinard, Julie A.;Grenier, Guillaume;Vermette, Patrick
通讯作者：
Vermette, Patrick
DOI：
10.1073/pnas.96.17.9815
发表时间：
1999-08-17
影响因子：
11.1
作者：
Corada, M;Mariotti, M;Dejana, E
通讯作者：
Dejana, E
影响因子：
21.3
作者：
Balaban, NQ;Schwarz, US;Geiger, B
通讯作者：
Geiger, B