Genome sequencing reveals diversification of virulence factor content and possible host adaptation in distinct subpopulations of Salmonella enterica.
Genome sequencing reveals diversification of virulence factor content and possible host adaptation in distinct subpopulations of Salmonella enterica.
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DOI：
10.1186/1471-2164-12-425
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发表时间：
2011-08-22
期刊：
影响因子：
4.4
通讯作者：
Wiedmann M
中科院分区：
文献类型：
--
作者：
den Bakker HC;Moreno Switt AI;Govoni G;Cummings CA;Ranieri ML;Degoricija L;Hoelzer K;Rodriguez-Rivera LD;Brown S;Bolchacova E;Furtado MR;Wiedmann M
细菌种群分化为不同的亚群通常是生态隔离的结果。虽然一些研究表明存在沙门氏菌肠道亚种。enterica subclades，这些细分的证据一直模棱两可。在这里，我们使用了比较基因组学的方法来确定肠道沙门氏菌亚种的人口结构。enterica，并确定分支特异性基因，可能是生态专业化的结果。多位点序列分析（MLSA）和单核苷酸多态性（SNPs）数据的16个新测序和30个公开可用的基因组显示了明确的细分S。菌肠道亚种肠杆菌分成至少两个亚群，我们称之为进化枝A和进化枝B。进化枝B菌株含有几个进化枝特异性基因或操纵子，包括β-葡萄糖醛酸酶操纵子、S-菌毛操纵子和细胞表面相关基因，这强烈表明该亚群的生态位特化。通过使用靶向亚群特异性SNP和目标基因的qPCR测定，将另外一组123个分离株分配到进化枝A和B。在检测的98个血清型中，约20%属于分支B。所有进化枝B分离株都含有两个致病性相关的基因组岛，SPI-18和一个细胞致死膨胀毒素岛;这两个岛的组合以前被认为是伤寒和甲型副伤寒血清型所独有的。进化枝B分离株中β-葡萄糖醛酸苷酶的存在特别表明该进化枝对脊椎动物胃肠道环境的适应。S.菌肠道亚种肠杆菌由至少两个亚群组成，所述亚群在涉及宿主和组织向性、利用宿主特异性碳源和氮源的基因上特异性不同，因此可能在生态学和传播特性上不同。
Divergence of bacterial populations into distinct subpopulations is often the result of ecological isolation. While some studies have suggested the existence of Salmonella enterica subsp. enterica subclades, evidence for these subdivisions has been ambiguous. Here we used a comparative genomics approach to define the population structure of Salmonella enterica subsp. enterica, and identify clade-specific genes that may be the result of ecological specialization. Multi-locus sequence analysis (MLSA) and single nucleotide polymorphisms (SNPs) data for 16 newly sequenced and 30 publicly available genomes showed an unambiguous subdivision of S. enterica subsp. enterica into at least two subpopulations, which we refer to as clade A and clade B. Clade B strains contain several clade-specific genes or operons, including a β-glucuronidase operon, a S-fimbrial operon, and cell surface related genes, which strongly suggests niche specialization of this subpopulation. An additional set of 123 isolates was assigned to clades A and B by using qPCR assays targeting subpopulation-specific SNPs and genes of interest. Among 98 serovars examined, approximately 20% belonged to clade B. All clade B isolates contained two pathogenicity related genomic islands, SPI-18 and a cytolethal distending toxin islet; a combination of these two islands was previously thought to be exclusive to serovars Typhi and Paratyphi A. Presence of β-glucuronidase in clade B isolates specifically suggests an adaptation of this clade to the vertebrate gastrointestinal environment. S. enterica subsp. enterica consists of at least two subpopulations that differ specifically in genes involved in host and tissue tropism, utilization of host specific carbon and nitrogen sources and are therefore likely to differ in ecology and transmission characteristics.
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影响因子：
5.8
作者：
Pond, Sergei L. Kosakovsky;Posada, David;Frost, Simon D. W.
通讯作者：
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影响因子：
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作者：
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Daigle, France
影响因子：
4.5
作者：
Didelot X;Bowden R;Street T;Golubchik T;Spencer C;McVean G;Sangal V;Anjum MF;Achtman M;Falush D;Donnelly P
通讯作者：
Donnelly P