Activin A‐induced diffrentiation in K562 cells is associated with a transient hypophosphorylation of RB protein and the concomitant block of cell cycle at G1 phase
Activin A‐induced diffrentiation in K562 cells is associated with a transient hypophosphorylation of RB protein and the concomitant block of cell cycle at G1 phase
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激活素 A 诱导的 K562 细胞分化与 RB 蛋白的短暂低磷酸化以及伴随的 G1 期细胞周期阻滞有关
DOI：
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发表时间：
1992
影响因子：
4
通讯作者：
A. Yu
中科院分区：
文献类型：
--
作者：
D. Sehy;L. Shao;W. Tsai;John Yu;A. Yu
人红白血病细胞系K562可通过加入激活素A诱导分化，激活素A是一种新纯化的转化生长因子-β1家族蛋白。本研究采用流式细胞仪细胞周期分析、增殖细胞核抗原间接免疫荧光染色和细胞增殖的胸腺嘧啶核苷掺入法研究激活素A对K652细胞分化过程中细胞周期的影响。激活素阶段。后者可在与激活素A孵育约24小时后观察到，然后在诱导分化的早期阶段消失。用同步化的K562细胞进行的细胞周期动力学分析也证实，在激活素A存在的情况下，K562细胞在细胞的不同时相中正常进行，只是在10-24小时培养的G1期有所延长。此外，G1期的这种短暂停滞与核蛋白的去磷酸化有关，核蛋白是Rb基因的产物，发生在与激活素A孵育的9-24小时内；随后Rb蛋白的磷酸化发展。此外，观察到这些与细胞周期相关的事件发生在K562细胞中的血红蛋白积累之前。结论：激活素A和化学诱导剂诱导红系分化的过程中，Rb蛋白瞬时去磷酸化和细胞周期G1期延长先于并伴随着红系分化，是红白血病细胞诱导分化的部分机制。©1992 Wiley-Liss，Inc.
The human erythroleukemic cell line, K562, can be induced to differentiate by the addition of activin A, a newly purified protein belonging to the TGF‐β1 family. The present studies used flow cytometric cell cycle analysis, indirect immunofluorescence staining of the proliferating cell nuclear antigen (PCNA), and thymidine incorporation assay of cell proliferation to study the effects of activin A on the cell cycle during differentiation in K652 cells. Activin phase. The latter can be observed after approximately 24 hr of incubation with activin A and then disappears after this early stage of induction of differentiation. Cell cycle kinetics analysis using synchronized K562 cells also confirms that in the presence of activin A, K562 cells progress normally through various phases of cell, except that there is prolongation of the G1 phase between 10 to 24 hr of culture. Furthermore, this transient arrest in G1 is correlated with dephosphorylation of a nucleoprotein, the RB gene product, which occurs within 9–24 hr of incubation with activin A; and phosphorylation of RB protein then develops afterward. In addition, these cell cycle‐related events are observed to occur earlier than the accumulation of hemoglobins in K562 cells. It is concluded that transient dephosphorylation of RB protein and prolongation of G1 phase of cell cycle precede and accompany erythroid differentiation caused by activin A and chemical inducers, thus constituting part of the mechanism for induction of differentiation in the erythroleukemia cells. © 1992 Wiley‐Liss, Inc.
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人白血病细胞系 K562 上激活素 A 结合位点的表征。
DOI：
10.1016/s0006-291x(88)80326-7
发表时间：
1988
影响因子：
3.1
作者：
Campen,CA;Vale,W
通讯作者：
Vale,W
人骨髓基质细胞和单核细胞中激活素 A 产生的调节。
影响因子：
2.6
作者：
Shao,L;FrigonJr,NL;Sehy,DW;Yu,AL;Lofgren,J;Schwall,R;Yu,J
通讯作者：
Yu,J
激活素对体外人红细胞集落形成的增强作用的表征。
影响因子：
20.3
作者：
Yu,J;Shao,L;Vaughan,J;Vale,W;Yu,AL
通讯作者：
Yu,AL
激活素 A 对纯化的人红系祖细胞中珠蛋白基因表达的影响。
影响因子：
20.3
作者：
Shao,L;FrigonJr,NL;Young,AL;Yu,AL;Mathews,LS;Vaughan,J;Vale,W;Yu,J
通讯作者：
Yu,J