Molecular Signals Involved in Human B Cell Migration into the Retina: In Vitro Investigation of ICAM-1, VCAM-1, and CXCL13.
Molecular Signals Involved in Human B Cell Migration into the Retina: In Vitro Investigation of ICAM-1, VCAM-1, and CXCL13.
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DOI：
10.1080/09273948.2016.1180401
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发表时间：
2017-12
影响因子：
3.3
通讯作者：
Smith JR
中科院分区：
文献类型：
--
作者：
Bharadwaj AS;Stempel AJ;Olivas A;Franzese SE;Ashander LM;Ma Y;Lie S;Appukuttan B;Smith JR
B细胞参与多种视网膜免疫病理过程。内皮细胞黏附分子和趋化因子引导白细胞的运输。我们研究了三种分子信号在人B细胞视网膜内皮细胞迁移中的作用：ICAM-1、VCAM-1和CXCL13。阴性选择法分离外周血B细胞。使用抗体阻断ICAM-1或VCAM-1，在填充了人视网膜内皮单分子层的跨孔中进行了迁移研究。观察CXCL13在视网膜中的表达。B细胞穿过视网膜内皮细胞。当所有受试者的结果综合在一起时，ICAM-1阻断显著减少了迁移，而当结果按个体分析时，对大多数人来说是如此。这种影响与CXCL13的存在与否无关，尽管CXCL13增加了迁移。CXCL13在神经视网膜和视网膜色素上皮中均有表达。部分视网膜血管内皮细胞表达CXCL13蛋白。ICAM-1阻断可能是治疗一些涉及B细胞的视网膜疾病的有效方法。
B cells participate in diverse retinal immunopathologies. Endothelial adhesion molecules and chemokines direct leukocyte trafficking. We examined the involvement of three molecular signals in retinal transendothelial migration of human B cells: ICAM-1, VCAM-1 and CXCL13. Peripheral blood B cells were isolated by negative selection. Migration was studied in transwells populated with human retinal endothelial monolayers, using antibody to block ICAM-1 or VCAM-1. Retinal expression of CXCL13 was investigated. B cells crossed retinal endothelium. ICAM-1 blockade significantly reduced migration when results for all subjects were combined, and for a majority when results were analyzed by individual. This effect was irrespective of the presence or absence of CXCL13, although CXCL13 increased migration. CXCL13 was detected in neural retina and retinal pigment epithelium. Endothelial cells of some retinal vessels presented CXCL13 protein. ICAM-1 blockade may be an effective treatment in some patients with retinal diseases that involve B cells.
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