Altered synthesis of genes associated with short-chain fatty acids in the gut of patients with atrial fibrillation.
Altered synthesis of genes associated with short-chain fatty acids in the gut of patients with atrial fibrillation.
复制标题
心房颤动患者肠道中与短链脂肪酸相关的基因合成发生改变。
DOI：
10.1186/s12864-021-07944-0
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发表时间：
2021-08-31
期刊：
影响因子：
4.4
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Zhang J;Zuo K;Fang C;Yin X;Liu X;Zhong J;Li K;Li J;Xu L;Yang X
肠道微生物群通过产生短链脂肪酸（SCFA）为人类提供健康益处，短链脂肪酸的缺乏会导致多种疾病和炎症性疾病。然而，心房颤动（AF）患者的肠道细菌产生SCFA，这是一种患病率不断增加的心律失常，尚未报道。为了研究与SCFA合成相关的主要肠道微生物，在包括50名非AF对照和50名AF患者的北方中国队列中，根据宏基因组数据挖掘检查SCFA相关KEGG直系同源物（科斯）、酶基因和潜在生产者。与非AF对照组相比，AF个体参与SCFA相关合成的微生物基因存在显著差异，包括125个科斯和5个SCFA相关酶基因。此外，有10种携带SCFA合成相关酶基因，并在AF患者的肠道中显着减少。值得注意的是，关于SCFA合成相关功能的判别特征，包括8个科斯（K 01752、K 01738、K 00175、K 03737、K 01006、K 01653、K 01647和K15023），4个基因（menI、tesB、yciA和CO脱氢酶乙酰辅酶A合酶复合物）和2种（粪球菌卡图斯和厚壁菌门细菌CAG：103）作为关键因子。此外，PLS-SEM分析表明，对肠道微生物群多样性和关键物种对AF的总体影响中，分别有72.8%和91.14%是由关键科斯介导的。同时，SCFA合成相关功能对左房扩大的总影响中，46.31%是由hsCRP介导的。合并AF的临床特征后，KO评分仍与AF发生率显著相关（OR = 0.004，P = 0.001）。目前的研究表明，AF中的生态失调肠道微生物群与SCFA合成相关基因的破坏相结合，其特征在于KEGG直系同源物、合成酶基因和窝藏物种的丰度降低。在线版本包含补充材料，可通过10.1186/s12864-021-07944-0获得。
The gut microbiota provides health benefits in humans by producing short-chain fatty acids (SCFAs), whose deficiency causes multiple disorders and inflammatory diseases. However, gut bacteria producing SCFAs in patients with atrial fibrillation (AF), an arrhythmia with increasing prevalence, have not been reported. To investigate major gut microbial organisms related to SCFA synthesis, SCFAs-associated KEGG orthologues (KOs), enzymatic genes, and potential producers were examined according to metagenomic data-mining in a northern Chinese cohort comprising 50 non-AF control and 50 AF patients. Compared with non-AF controls, individuals with AF had marked differences in microbial genes involved in SCFA-related synthesis, including 125 KOs and 5 SCFAs-related enzymatic genes. Furthermore, there were 10 species that harbored SCFA-synthesis related enzymatic genes, and were markedly decreased in the gut of AF patients. Notably, discriminative features about SCFA-synthesis related function, including 8 KOs (K01752, K01738, K00175, K03737, K01006, K01653, K01647 and K15023), 4 genes (menI, tesB, yciA and CO dehydrogenase acetyl-CoA synthase complex) and 2 species (Coprococcus catus and Firmicutes bacterium CAG:103), were selected as key factors based on LASSO analysis. Furthermore, PLS-SEM analysis showed that 72.8 and 91.14 % of the overall effects on gut microbiota diversity and key species on AF, respectively, were mediated by the key KOs. Meanwhile, 46.31 % of the total effects of SCFA-synthesis related function on left atrial enlargement was mediated by hsCRP. Upon incorporation of clinical properties in AF, the KO score was still significantly associated with AF incidence (OR = 0.004, P = 0.001). The current study revealed that dysbiotic gut microbiota in AF is coupled with disrupted SCFA-synthesis related genes, characterized by decreased abundances of KEGG orthologues, synthesis enzymatic genes and harboring species. The online version contains supplementary material available at 10.1186/s12864-021-07944-0.
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影响因子：
64.8
作者：
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影响因子：
64.5
作者：
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影响因子：
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DOI：
10.1073/pnas.1116053109
发表时间：
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