Fish oil diet affects on oxidative senescence of red blood cells linked to degeneration of spleen cells in mice.
Fish oil diet affects on oxidative senescence of red blood cells linked to degeneration of spleen cells in mice.
复制标题
鱼油饮食会影响与小鼠脾细胞变性相关的红细胞氧化衰老。
DOI：
10.1016/s1388-1981(00)00076-7
复制
发表时间：
2000
期刊：
影响因子：
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通讯作者：
T. Miyazawa
中科院分区：
文献类型：
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作者：
M. Oarada;Hiroshi Furukawa;Toshiro Majima;T. Miyazawa
采用富含二十碳五烯酸（EPA, 20:5, n-3）和二十二碳六烯酸（DHA, 22:6, n-3）的鱼油（15%油/日粮，w/w），比较研究了饲粮中添加多不饱和脂肪酸和α-生育酚对小鼠红细胞脂质过氧化和免疫活性细胞的影响。与大豆油喂养的小鼠相比，鱼油喂养30天的小鼠脾脏重量增加43%，脾脏细胞数量增加约一倍，脾脏细胞亚群没有变化，脾脏中有丝分裂原诱导的T细胞和B细胞的形成明显减少。在鱼油喂养的小鼠中，作为红细胞氧化衰老标志的膜脂过氧化氢（磷脂酰胆碱和磷脂酰乙醇胺的过氧化氢）积累比大豆油喂养的小鼠高2.7-3.5倍，尽管血浆磷脂酰胆碱过氧化氢浓度没有差异。尽管α-生育酚的添加量高达基础饲料的10倍，但仍不能阻止鱼油喂养引起的脾脏细胞退化和红细胞氧化衰老。结果表明，在鱼油饲料中口服过量的多不饱和脂肪酸，如EPA和DHA，可导致膜脂过氧化加速，导致红细胞衰老，与脾脏细胞免疫反应降低有关，而α-生育酚作为抗氧化剂的补充并不总是有效地防止脾脏细胞和红细胞的氧化变性。
The effect of dietary polyunsaturated fatty acids and α-tocopherol supplementation on erythrocyte lipid peroxidation and immunocompetent cells in mice was studied comparatively using seven dietary oils (15% oil/diet, w/w) including fish oil rich in eicosapentaenoic acid (EPA, 20:5, n–3) and docosahexaenoic acid (DHA, 22:6, n–3). A 43% increase in spleen weight, about twice as many spleen cells and no change in the subpopulations of spleen cells, as well as a significant depression of mitogen-induced blastogenesis of both T and B cells in the spleen were observed in mice fed fish oil for 30 days in comparison with soybean oil diet-fed mice. In the fish oil diet-fed mice, membranous lipid hydroperoxide (hydroperoxides of phosphatidylcholine and phosphatidylethanolamine) accumulation as a marker of oxidative senescence in red blood cells (RBC) was 2.7–3.5 times higher than that in mice fed soybean oil, although there was no difference in the plasma phosphatidylcholine hydroperoxide concentration. In spite of the supplementation of α-tocopherol to up to 10 times the level in the basal diet, the degeneration of spleen cells and the stimulated oxidative senescence of RBC found by the fish oil feeding could not be prevented. The results suggest that oral intake of excess polyunsaturated fatty acids, i.e. EPA and DHA, in a fish oil diet can lead to acceleration of membrane lipid peroxidation resulting in RBC senescence linked to the lowering of immune response of spleen cells, and that supplementation of α-tocopherol as antioxidant does not always effectively prevent such oxidative degeneration as observed in spleen cells and RBC in vivo.
影响因子：
4.2
作者：
MEYDANI, M;NATIELLO, F;GORBACH, SL
通讯作者：
GORBACH, SL
n-3 脂肪酸和人类高血压。
影响因子：
4.4
作者：
Knapp,HR
通讯作者：
Knapp,HR
荧光激活细胞分选：理论、实验优化以及在淋巴细胞生物学中的应用。
影响因子：
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作者：
Parks,DR;Herzenberg,LA
通讯作者：
Herzenberg,LA