Cross-Talk between the Aeromonas hydrophila Type III Secretion System and Lateral Flagella System.
Cross-Talk between the Aeromonas hydrophila Type III Secretion System and Lateral Flagella System.
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DOI：
10.3389/fmicb.2016.01434
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发表时间：
2016
影响因子：
5.2
通讯作者：
Shaw JG
中科院分区：
文献类型：
--
作者：
Zhao YH;Shaw JG
嗜水气单胞菌导致鱼类的气单胞菌败血症，以及人类的肠胃炎和伤口感染。气单胞菌利用III型分泌系统（T3SS）将蛋白效应器直接注射到宿主细胞中。几种细菌中T3SS的主要遗传调控因子之一是arac样蛋白ExsA。先前的研究表明T3SS调控与侧鞭毛表达之间存在联系。本研究的目的是确定T3SS的遗传调控及其与嗜水草侧鞭毛系统的潜在相互作用。为了研究编码T3SS调控组分的基因exsA、exsD、exsC和exsE的突变，并在野生型和突变背景下测量了T3SS启动子的活性，证明了一个调控网络。通过BACTH实验和Far-Western Blot发现Exs蛋白之间存在相互作用。这些发现提示了一个调控级联，其中ExsE与伴侣蛋白ExsC结合。当ExsC游离时，它会隔离抗激活剂ExsD，从而停止对T3SS主调控因子ExsA的抑制，从而允许T3SS表达。T3SS调控组分也影响外侧鞭毛系统的表达。结果表明，由于缺乏ExsD和ExsE，外侧鞭毛启动子的活性受到抑制，这表明T3SS主调控因子ExsA是外侧鞭毛系统的负调控因子。
Aeromonas hydrophila is responsible for aeromonad septicaemia in fish, and gastroenteritis and wound infections in humans. The type III secretion system (T3SS) is utilized by aeromonads to inject protein effectors directly into host cells. One of the major genetic regulators of the T3SS in several bacterial species is the AraC-like protein ExsA. Previous studies have suggested a link between T3SS regulation and lateral flagella expression. The aim of this study was to determine the genetic regulation of the T3SS and its potential interaction with the lateral flagella system in A. hydrophila. To investigate the genes encoding the T3SS regulatory components exsA, exsD, exsC, and exsE were mutated and the activities of the T3SS promoters were measured in wild type and mutant backgrounds demonstrating a regulatory network. The Exs proteins were shown to interact with each other by BACTH assay and Far-Western Blot. The findings suggested a regulatory cascade in which ExsE was bound to the chaperone protein ExsC. When ExsC was free it sequestered the anti-activator ExsD thus stopping the inhibition of the T3SS master regulator ExsA allowing T3SS expression. The T3SS regulatory components were also shown to affect the expression of the lateral flagella system. The activities of the lateral flagella promoters were shown to be repressed by the absence of ExsD and ExsE, suggesting that the T3SS master regulator ExsA was a negative regulator of the lateral flagella system.
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影响因子：
5.2
作者：
Diaz MR;King JM;Yahr TL
通讯作者：
Yahr TL
影响因子：
4.5
作者：
Abby SS;Rocha EP
通讯作者：
Rocha EP
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
3.6
作者：
Dasgupta, N;Lykken, GL;Yahr, TL
通讯作者：
Yahr, TL
DOI：
10.1111/febs.12103
发表时间：
2013-02
期刊：
The FEBS journal
影响因子：
--
作者：
Bernhards RC;Marsden AE;Esher SK;Yahr TL;Schubot FD
通讯作者：
Schubot FD