Extracellular Vesicles as Potential Biomarkers for Early Detection and Diagnosis of Pancreatic Cancer.
Extracellular Vesicles as Potential Biomarkers for Early Detection and Diagnosis of Pancreatic Cancer.
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DOI：
10.3390/biomedicines8120581
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发表时间：
2020-12-07
期刊：
影响因子：
4.7
通讯作者：
Zheng SY
中科院分区：
文献类型：
--
作者：
Yee NS;Zhang S;He HZ;Zheng SY
胰腺癌（PC）是高度转移性的，并且它倾向于在晚期被检测到。识别和开发用于早期检测PC的生物标志物对于潜在的治愈性治疗至关重要。细胞外囊泡是一种存在于人体体液中的双层脂质膜结构的纳米囊泡，在肿瘤的生物发生和转移中发挥着重要作用。癌症衍生的EV富含DNA、RNA、蛋白质和脂质，它们已成为早期检测PC的有吸引力的诊断生物标志物。在这篇文章中，我们概述了EV的细胞生物学及其分离和分析，以及它们在癌症发病机制和进展中的作用。对基于血浆的外泌体的基因组DNA、microRNA、mRNA、环状RNA和蛋白质的多平台分析用于PC的诊断进行了严格的审查。大量证据表明，分析基于EV的生物标志物的液体活检对PC的诊断具有不同的性能。未来的研究表明，优化的方法，分离和分析EV，并确定基于EV的生物标志物和其他临床数据集的组合，以提高预测值，灵敏度和特异性的筛选测试PC的早期检测和诊断的目标。
Pancreatic carcinoma (PC) is highly metastatic, and it tends to be detected at advanced stages. Identifying and developing biomarkers for early detection of PC is crucial for a potentially curative treatment. Extracellular vesicles (EVs) are bilayer lipid membrane-structured nanovesicles found in various human bodily fluids, and they play important roles in tumor biogenesis and metastasis. Cancer-derived EVs are enriched with DNA, RNA, protein, and lipid, and they have emerged as attractive diagnostic biomarkers for early detection of PC. In this article, we provided an overview of the cell biology of EVs and their isolation and analysis, and their roles in cancer pathogenesis and progression. Multiplatform analyses of plasma-based exosomes for genomic DNA, micro RNA, mRNA, circular RNA, and protein for diagnosis of PC were critically reviewed. Numerous lines of evidence demonstrate that liquid biopsy with analysis of EV-based biomarkers has variable performance for diagnosis of PC. Future investigation is indicated to optimize the methodology for isolating and analyzing EVs and to identify the combination of EV-based biomarkers and other clinical datasets, with the goal of improving the predictive value, sensitivity, and specificity of screening tests for early detection and diagnosis of PC.
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影响因子：
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