An elevated expression of serum exosomal microRNA-191, - 21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker.
An elevated expression of serum exosomal microRNA-191, - 21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker.
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DOI：
10.1186/s12885-018-4006-5
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发表时间：
2018-01-31
期刊：
影响因子：
3.8
通讯作者：
Okumura T
中科院分区：
文献类型：
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作者：
Goto T;Fujiya M;Konishi H;Sasajima J;Fujibayashi S;Hayashi A;Utsumi T;Sato H;Iwama T;Ijiri M;Sakatani A;Tanaka K;Nomura Y;Ueno N;Kashima S;Moriichi K;Mizukami Y;Kohgo Y;Okumura T
胰腺癌的预后极差，因此迫切需要能够检测初始阶段的新生物标志物。血清microRNA（miR）水平在胰腺肿瘤如胰腺癌和导管内乳头状粘液性肿瘤（IPMN）诊断中的意义尚不清楚。我们在此评估了包封在血清外泌体（ExmiR）中的miR作为诊断标志物的有用性。使用ExoQuick-TC™富集来自患有胰腺癌（n = 32）或IPMN（n = 29）的患者和没有肿瘤的患者（对照; n = 22）的ExmiR。使用下一代测序分析评估ExmiR的表达，并通过定量实时聚合酶链反应确认通过该分析选择的三种miR。ExmiR-191、ExmiR-21和ExmiR-451 a在胰腺癌和IPMN患者中的表达与对照相比显著上调（p < 0.05）。受试者操作特征曲线分析显示，ExmiR的曲线下面积和诊断准确性上级三种血清体积循环miR（例如; ExmiR-21：AUC 0.826，准确率80.8%。循环miR-21：AUC 0.653，准确度62.3%）。此外，高ExmiR-451 a与IPMN中的壁结节相关（p = 0.010），高ExmiR-21被鉴定为总生存期（p = 0.011，HR 4.071，高ExmiR-21的中位OS：344天，低ExmiR-21的中位OS：846天）和化学抗性标志物（p = 0.022）的候选预后因素。因此，三种ExmiR的水平可以用作胰腺癌和IPMN的早期诊断和进展标志物，并且被认为比循环miR（限于这三种miR）更有用的标志物。本文的在线版本（10.1186/s12885-018-4006-5）包含补充材料，可供授权用户使用。
Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers. The ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™. The expression of ExmiRs was evaluated using a next-generation sequencing analysis, and the selected three miRs through this analysis were confirmed by a quantitative real-time polymerase chain reaction. The expression of ExmiR-191, ExmiR-21 and ExmiR-451a was significantly up-regulated in patients with pancreatic cancer and IPMN compared to the controls (p < 0.05). A receiver operating characteristic curve analysis showed that the area under the curve and the diagnostic accuracy of ExmiRs were 5–20% superior to those of three serum bulky circulating miRs (e.g.; ExmiR-21: AUC 0.826, accuracy 80.8%. Circulating miR-21: AUC 0.653, accuracy 62.3%). In addition, high ExmiR-451a was associated with mural nodules in IPMN (p = 0.010), and high ExmiR-21 was identified as a candidate prognostic factor for the overall survival (p = 0.011, HR 4.071, median OS of high-ExmiR-21: 344 days, median OS of low-ExmiR-21: 846 days) and chemo-resistant markers (p = 0.022). The level of three ExmiRs can thus serve as early diagnostic and progression markers of pancreatic cancer and IPMN, and considered more useful markers than the circulating miRs (limited to these three miRs). The online version of this article (10.1186/s12885-018-4006-5) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
Zhang XF;Li KK;Gao L;Li SZ;Chen K;Zhang JB;Wang D;Tu RF;Zhang JX;Tao KX;Wang G;Zhang XD
通讯作者：
Zhang XD
影响因子：
3.7
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影响因子：
254.7
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通讯作者：
Ward, Elizabeth
影响因子：
3.7
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通讯作者：
Piiper A
影响因子：
2.9
作者：
Maguchi, Hiroyuki;Tanno, Satoshi;Tanaka, Masao
通讯作者：
Tanaka, Masao