A Different Microbiome Gene Repertoire in the Airways of Cystic Fibrosis Patients with Severe Lung Disease.
A Different Microbiome Gene Repertoire in the Airways of Cystic Fibrosis Patients with Severe Lung Disease.
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DOI：
10.3390/ijms18081654
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发表时间：
2017-07-29
影响因子：
5.6
通讯作者：
Bevivino A
中科院分区：
文献类型：
--
作者：
Bacci G;Mengoni A;Fiscarelli E;Segata N;Taccetti G;Dolce D;Paganin P;Morelli P;Tuccio V;De Alessandri A;Lucidi V;Bevivino A
近年来，下一代测序技术(NGS)被用于破译囊性纤维化(CF)患者呼吸道微生物区系的结构和组成。然而，关于常驻微生物群体所具有的全部基因功能以及哪些特定基因与不同阶段的CF肺部疾病相关的信息仍然很少。在本研究中，我们旨在通过痰散弹枪元基因组测序来确定12例重症和正常/轻度肺部疾病患者的CF微生物群的微生物基因库。从元基因组中重建了由栖息在CF呼吸道的微生物编码的代谢途径的丰度。我们发现了一组在不同肺功能患者中不同分布的代谢途径，即与细菌趋化和鞭毛组装相关的途径，以及编码外排介导的抗生素耐药机制的基因和毒力相关基因。结果表明，肺功能低下患者的微生物群富含毒力相关基因和编码外排介导的抗生素耐药机制的基因。总体而言，受严重影响的成人CF的微生物群落似乎编码了促进微生物在肺内定植和持续存在的不同机制，这与严重肺部疾病患者常见的多药耐药微生物群落的特征一致。
In recent years, next-generation sequencing (NGS) was employed to decipher the structure and composition of the microbiota of the airways in cystic fibrosis (CF) patients. However, little is still known about the overall gene functions harbored by the resident microbial populations and which specific genes are associated with various stages of CF lung disease. In the present study, we aimed to identify the microbial gene repertoire of CF microbiota in twelve patients with severe and normal/mild lung disease by performing sputum shotgun metagenome sequencing. The abundance of metabolic pathways encoded by microbes inhabiting CF airways was reconstructed from the metagenome. We identified a set of metabolic pathways differently distributed in patients with different pulmonary function; namely, pathways related to bacterial chemotaxis and flagellar assembly, as well as genes encoding efflux-mediated antibiotic resistance mechanisms and virulence-related genes. The results indicated that the microbiome of CF patients with low pulmonary function is enriched in virulence-related genes and in genes encoding efflux-mediated antibiotic resistance mechanisms. Overall, the microbiome of severely affected adults with CF seems to encode different mechanisms for the facilitation of microbial colonization and persistence in the lung, consistent with the characteristics of multidrug-resistant microbial communities that are commonly observed in patients with severe lung disease.
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影响因子：
7.7
作者：
Bacci, G.;Bazzicalupo, M.;Mengoni, A.
通讯作者：
Mengoni, A.
影响因子：
3.7
作者：
Bacci, Giovanni;Paganin, Patrizia;Mengoni, Alessio
通讯作者：
Mengoni, Alessio
影响因子：
4.4
作者：
Chawley, Parmita;Samal, Himanshu Bhusan;Mahapatra, Rajani Kanta
通讯作者：
Mahapatra, Rajani Kanta
影响因子：
18.2
作者：
Dickson RP;Erb-Downward JR;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
在囊性纤维化中，跨年龄和疾病阶段的肺微生物群。
影响因子：
4.6
作者：
Coburn B;Wang PW;Diaz Caballero J;Clark ST;Brahma V;Donaldson S;Zhang Y;Surendra A;Gong Y;Elizabeth Tullis D;Yau YC;Waters VJ;Hwang DM;Guttman DS
通讯作者：
Guttman DS