Reciprocal connectivity between mitral cells and external plexiform layer interneurons in the mouse olfactory bulb.
Reciprocal connectivity between mitral cells and external plexiform layer interneurons in the mouse olfactory bulb.
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DOI：
10.3389/fncir.2013.00032
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发表时间：
2013
影响因子：
3.5
通讯作者：
Arenkiel BR
中科院分区：
文献类型：
--
作者：
Huang L;Garcia I;Jen HI;Arenkiel BR
适当的大脑功能依赖于兴奋和抑制之间的微妙平衡，其中抑制回路在塑造神经元输出和信息处理的原则方面发挥着重要作用。在嗅球回路的突出模型中，局部中间神经元对二尖瓣细胞的抑制使气味调谐变得尖锐，并提供对比度增强。僧帽细胞抑制发生在僧帽细胞顶端树突和颗粒细胞之间的深层树突-树突突触，颗粒细胞是嗅球中最丰富的抑制性中间神经元群体。然而，目前尚不清楚其他局部中间神经元是否与二尖瓣细胞产生抑制性连接。在这里，我们报告了一种新的电路与强大的和相互连接的亚群以前未表征的促肾上腺皮质激素释放激素（CRH）表达的中间神经元位于外网层（EPL），和二尖瓣细胞。使用细胞类型特异性遗传操作，成像，光遗传学刺激和电生理记录，我们揭示了CRH表达的EPL中间神经元强烈抑制二尖瓣细胞放电，并且它们被快速的神经元兴奋的二尖瓣细胞输入。这些研究结果在功能上确定了一种新的嗅球中间神经元亚群，该亚群显示与二尖瓣细胞的相互连接，揭示了一种以前未知的，可能影响横向相互作用和/或促进气味处理的嗅球回路中的潜在关键球员。
Proper brain function relies on exquisite balance between excitation and inhibition, where inhibitory circuits play fundamental roles toward sculpting principle neuron output and information processing. In prominent models of olfactory bulb circuitry, inhibition of mitral cells by local interneurons sharpens odor tuning and provides contrast enhancement. Mitral cell inhibition occurs at both mitral cell apical dendrites and deep-layer dendrodendritic synapses between granule cells, the most abundant population of inhibitory interneurons in the olfactory bulb. However, it remains unclear whether other local interneurons make inhibitory connections onto mitral cells. Here, we report a novel circuitry with strong and reciprocal connectivity between a subpopulation of previously uncharacterized Corticotropin-Releasing Hormone (CRH)-expressing interneurons located in the external plexiform layer (EPL), and mitral cells. Using cell type-specific genetic manipulations, imaging, optogenetic stimulation, and electrophysiological recordings, we reveal that CRH-expressing EPL interneurons strongly inhibit mitral cell firing, and that they are reciprocally excited by fast glutamatergic mitral cell input. These findings functionally identify a novel subpopulation of olfactory bulb interneurons that show reciprocal connectivity with mitral cells, uncovering a previously unknown, and potentially critical player in olfactory bulb circuitry that may influence lateral interactions and/or facilitate odor processing.
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影响因子：
64.8
作者：
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通讯作者：
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作者：
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