An Induced Mutation in HvRECQL4 Increases the Overall Recombination and Restores Fertility in a Barley HvMLH3 Mutant Background.
An Induced Mutation in HvRECQL4 Increases the Overall Recombination and Restores Fertility in a Barley HvMLH3 Mutant Background.
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DOI：
10.3389/fpls.2021.706560
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发表时间：
2021
影响因子：
5.6
通讯作者：
Ramsay L
中科院分区：
文献类型：
--
作者：
Arrieta M;Macaulay M;Colas I;Schreiber M;Shaw PD;Waugh R;Ramsay L
植物育种依靠减数分裂的重组或交换，产生新的等位基因组合沿着和染色体之间。然而，在作物如大麦中，由于低频率和偏态分布的组合，杂交受到限制。在这项研究中，我们试图确定限制重组的基因进行抑制筛选恢复生育能力的半可育大麦突变体desynaptic 10（des 10），携带突变的大麦直系同源物的MutL同源物3（HvMLH 3），一个成员的MutL同源物（MLH）家族的DNA错配修复基因。des 10突变体表现出减少的重组和更少的交叉，导致专性交换（CO）的丧失，从而导致染色体错误分离。我们确定了几个候选抑制系，并确认其恢复生育力的Hvmlh 3背景下，在随后的几代。我们专注于候选抑制系之一，SuppLine 2099，这表明最完全的恢复生育。我们通过使用靶序列富集和测序（TENSEQ）捕获阵列代表大麦直向同源物的46个减数分裂基因的特点这条线。我们发现SuppLine 2099在抗CO基因RecQ样解旋酶4（RECQL 4）中含有C/T改变，导致保守解旋酶结构域中的非极性甘氨酸被极性天冬氨酸（G700 D）氨基酸取代。F3群体的单核苷酸多态性（SNP）基因分型揭示了在Hvmlh 3背景中具有Hvrecql 4的品系中重组频率的显著增加，这与生育力的恢复相关。基因分型还表明，与野生型（WT）相比，在纯合Hvrecql 4系中存在几乎两倍的重组水平。然而，我们没有观察到CO事件分布的任何显著变化。我们的研究结果证实了RECQL 4在大基因组谷物中的抗CO作用，并建立了在传统作物改良背景下测试增加重组效用的可能性。
Plant breeding relies on the meiotic recombination or crossing over to generate the new combinations of the alleles along and among the chromosomes. However, crossing over is constrained in the crops such as barley by a combination of the low frequency and biased distribution. In this study, we attempted to identify the genes that limit the recombination by performing a suppressor screen for the restoration of fertility to the semi-fertile barley mutant desynaptic10 (des10), carrying a mutation in the barley ortholog of MutL-Homolog 3 (HvMLH3), a member of the MutL-homolog (MLH) family of DNA mismatch repair genes. des10 mutants exhibit reduced recombination and fewer chiasmata, resulting in the loss of obligate crossovers (COs) leading to chromosome mis-segregation. We identified several candidate suppressor lines and confirmed their restored fertility in an Hvmlh3 background in the subsequent generations. We focus on one of the candidate suppressor lines, SuppLine2099, which showed the most complete restoration of fertility. We characterized this line by using a target-sequence enrichment and sequencing (TENSEQ) capture array representing barley orthologs of 46 meiotic genes. We found that SuppLine2099 contained a C/T change in the anti-CO gene RecQ-like helicase 4 (RECQL4) resulting in the substitution of a non-polar glycine to a polar aspartic acid (G700D) amino acid in the conserved helicase domain. Single nucleotide polymorphism (SNP) genotyping of F3 populations revealed a significant increase in the recombination frequency in lines with Hvrecql4 in the Hvmlh3 background that was associated with the restoration of fertility. The genotyping also indicated that there was nearly double the recombination levels in homozygous Hvrecql4 lines compared to the wild type (WT). However, we did not observe any significant change in the distribution of CO events. Our results confirm the anti-CO role of RECQL4 in a large genome cereal and establish the possibility of testing the utility of increasing recombination in the context of traditional crop improvement.
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影响因子：
4.5
作者：
Girard C;Chelysheva L;Choinard S;Froger N;Macaisne N;Lemhemdi A;Mazel J;Crismani W;Mercier R
通讯作者：
Mercier R
影响因子：
5.6
作者：
Bayer MM;Rapazote-Flores P;Ganal M;Hedley PE;Macaulay M;Plieske J;Ramsay L;Russell J;Shaw PD;Thomas W;Waugh R
通讯作者：
Waugh R
影响因子：
56.9
作者：
Crismani, Wayne;Girard, Chloe;Mercier, Raphael
通讯作者：
Mercier, Raphael
影响因子：
7.2
作者：
Caldwell, DG;McCallum, N;Waugh, R
通讯作者：
Waugh, R
DOI：
10.1007/978-3-662-44406-1_16
发表时间：
2014-01-01
期刊：
BIOTECHNOLOGICAL APPROACHES TO BARLEY IMPROVEMENT
影响因子：
--
作者：
Ramsay, Luke;Colas, Isabelle;Waugh, Robbie
通讯作者：
Waugh, Robbie