Investigating the link between molecular subtypes of glioblastoma, epithelial-mesenchymal transition, and CD133 cell surface protein.
Investigating the link between molecular subtypes of glioblastoma, epithelial-mesenchymal transition, and CD133 cell surface protein.
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DOI：
10.1371/journal.pone.0064169
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kohandel M
中科院分区：
文献类型：
--
作者：
Zarkoob H;Taube JH;Singh SK;Mani SA;Kohandel M
在这篇手稿中，我们使用遗传数据对胶质母细胞瘤、上皮向间充质转化(EMT)和CD133细胞表面蛋白之间的分子亚类之间的联系进行了三方面的分析。这篇论文的贡献有三个方面：首先，我们使用了一个新发现的人乳腺上皮细胞上皮向间充质转化的信号，并证明了该信号中的基因与所有已知GBM亚型中差异表达的基因有显著重叠。然而，在GBM间质亚型和EMT信号中上调的基因之间的重叠比其他GBM亚型更显著。第二，我们提供的证据表明，EMT的遗传签名与CD133细胞表面蛋白之间存在负相关，CD133细胞表面蛋白是神经干细胞的假定标记。第三，我们研究了GBM分子亚型与CD133细胞表面蛋白遗传标志的相关性。我们证明，间充质和神经亚型的GBM与CD133基因特征有最强的相关性。虽然间充质亚型与EMT和CD133的签名相似，但也显示出与这些签名的某些差异，部分原因是EMT和CD133的签名彼此相反。综上所述，这些数据揭示了间充质转化和神经干细胞在多形性胶质母细胞瘤分子亚型中的作用及其相互作用。
In this manuscript, we use genetic data to provide a three-faceted analysis on the links between molecular subclasses of glioblastoma, epithelial-to-mesenchymal transition (EMT) and CD133 cell surface protein. The contribution of this paper is three-fold: First, we use a newly identified signature for epithelial-to-mesenchymal transition in human mammary epithelial cells, and demonstrate that genes in this signature have significant overlap with genes differentially expressed in all known GBM subtypes. However, the overlap between genes up regulated in the mesenchymal subtype of GBM and in the EMT signature was more significant than other GBM subtypes. Second, we provide evidence that there is a negative correlation between the genetic signature of EMT and that of CD133 cell surface protein, a putative marker for neural stem cells. Third, we study the correlation between GBM molecular subtypes and the genetic signature of CD133 cell surface protein. We demonstrate that the mesenchymal and neural subtypes of GBM have the strongest correlations with the CD133 genetic signature. While the mesenchymal subtype of GBM displays similarity with the signatures of both EMT and CD133, it also exhibits some differences with each of these signatures that are partly due to the fact that the signatures of EMT and CD133 are inversely related to each other. Taken together these data shed light on the role of the mesenchymal transition and neural stem cells, and their mutual interaction, in molecular subtypes of glioblastoma multiforme.
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影响因子：
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Mikheeva SA;Mikheev AM;Petit A;Beyer R;Oxford RG;Khorasani L;Maxwell JP;Glackin CA;Wakimoto H;González-Herrero I;Sánchez-García I;Silber JR;Horner PJ;Rostomily RC
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通讯作者：
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