Lymphocyte-independent connective tissue mast cells populate murine synovium.
Lymphocyte-independent connective tissue mast cells populate murine synovium.
复制标题
小鼠滑膜中分布着非淋巴细胞依赖性结缔组织肥大细胞。
DOI：
10.1002/art.22058
复制
发表时间：
2006
影响因子：
--
通讯作者：
Lee,DavidM
中科院分区：
文献类型：
--
作者：
Shin,Kichul;Gurish,MichaelF;Friend,DanielS;Pemberton,AlanD;Thornton,ElisabethM;Miller,HughR;Lee,DavidM
肥大细胞(Mast Cells，MC)是一种异质性的骨髓来源细胞群，不同的MC亚群位于特定的显微解剖位置。小鼠滑膜MC的表型尚不明确。由于MCs与炎性关节炎的发病机制有关，我们试图确定正常关节和关节炎关节中滑膜MC群体的表型。方法用免疫组织化学方法检测正常和K/BxN血清转移性关节炎滑膜组织中MC的表型。用组织形态计量学方法测定健康滑膜和关节炎滑膜中MC的数量和密度。结果正常滑膜组织和关节炎滑膜组织中的MC均呈结缔组织肥大细胞(CTMC)表型，表达mMCP-4、-5、-6和-7，但不表达mMCP-1和mMCP-2。在RAG基因缺失的小鼠中，MC增殖的表型和程度与正常小鼠中观察到的相同，无论是否患有关节炎。此外，与皮肤CTMC相比，所有滑膜MC都表达mMCP-6，这表明滑膜CTMC群体与其他解剖CTMC群体不同。结论我们的研究结果表明，小鼠滑膜MC群体由淋巴细胞无关的CTMC组成，并将关节炎滑膜识别为一个模型系统，通过该系统可以深入了解慢性炎症中CTMC的生理学机制。
ObjectiveMast cells (MCs) are a heterogeneous population of tissue‐resident bone marrow–derived cells; distinct MC subpopulations are situated at specific microanatomic locations. The phenotype of the murine synovial MC remains undefined. Since MCs have been implicated in the pathogenesis of inflammatory arthritis, we sought to define the phenotype of the murine synovial MC population in normal and arthritic joints. We also examined the contribution of lymphocytes to synovial MC physiology.MethodsThe MC phenotype in healthy and K/BxN serum transfer–induced arthritic synovial tissue was defined using immunohistochemical staining of prototypic MC‐specific proteases (murine MC proteases [mMCP] 1, 2, 4, 5, 6, and 7) (chymases and tryptases). MC numbers and density were determined by histomorphometry in healthy and arthritic synovia. The lymphocyte contribution to MC populations was assessed using RAG‐null mice.ResultsWe found that synovial MCs display a connective tissue mast cell (CTMC) phenotype in both normal and arthritic synovial tissue, which expresses mMCP‐4, ‐5, ‐6, and ‐7, but not mMCP‐1 or mMCP‐2. In addition, MC hyperplasia was seen in the arthritic synovium. In RAG‐null mice, the phenotype and degree of MC hyperplasia were identical to those observed in normal mice with and without arthritis. Furthermore, in contrast to skin CTMCs, all synovial MCs expressed mMCP‐6, demonstrating discrete differences between synovial CTMCs and other anatomic CTMC populations.ConclusionOur findings demonstrate that the murine synovial MC population is composed of lymphocyte‐independent CTMCs and identify arthritic synovium as a model system by which to gain insight into the poorly understood physiology of CTMCs in chronic inflammation.
登录
查看更多内容
DOI：
10.1084/jem.160.1.12
发表时间：
1984-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Guy-Grand D;Dy M;Luffau G;Vassalli P
通讯作者：
Vassalli P
影响因子：
15.9
作者：
Blair, RJ;Meng, H;Gruber, BL
通讯作者：
Gruber, BL
影响因子：
4.4
作者：
A. Irani;S. Craig;G. Deblois;C. Elson;N. Schechter;L. Schwartz
通讯作者：
A. Irani;S. Craig;G. Deblois;C. Elson;N. Schechter;L. Schwartz
腹膜内转移到肥大细胞中遗传缺陷的W/WV小鼠中后，骨髓衍生的肥大细胞的表型变化。
影响因子：
15.3
作者：
OTSU, K;NAKANO, T;KANAKURA, Y;ASAI, H;KATZ, HR;AUSTEN, KF;STEVENS, RL;GALLI, SJ;KITAMURA, Y
通讯作者：
KITAMURA, Y
小鼠肥大细胞蛋白酶 5 的翻译和颗粒定位。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
McNeil,HP;Frenkel,DP;Austen,KF;Friend,DS;Stevens,RL
通讯作者：
Stevens,RL