Relevance of MIC-1 in the Era of PSA as a Serum Based Predictor of Prostate Cancer: A Critical Evaluation.
Relevance of MIC-1 in the Era of PSA as a Serum Based Predictor of Prostate Cancer: A Critical Evaluation.
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DOI：
10.1038/s41598-017-17207-2
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发表时间：
2017-12-04
影响因子：
4.6
通讯作者：
Mandhani A
中科院分区：
文献类型：
--
作者：
Bansal N;Kumar D;Gupta A;Chandra D;Sankhwar SN;Mandhani A
为了减少不同研究中关于前列腺癌（PC）、良性前列腺增生（BPH）和健康对照（HC）血清中巨噬细胞抑制性细胞因子-1（MIC-1）表达水平的相互矛盾的观察结果的模糊性，我们设计了这项双盲研究。该研究包括来自PC、BPH和HC受试者的240份血清。采用Western blot和ELISA方法检测PC、BPH和HC中MIC-1的表达水平。WB和ELISA检测结果显示，PC中MIC-1的表达水平显著高于HC和BPH。回归分析显示MIC-1与PSA（r = 0.09; p < 0.001）和MIC-1与GS（r = 0.7; p < 0.001）之间存在显著相关性。ROC分析显示MIC-1优于PSA。此外，MIC-1和PSA的组合使得区分BPH + PC与HC的AUC为99.1%，区分BPH与HC的AUC为97.9%，区分PC与HC的AUC为98.6%，区分PC与BPH的AUC为96.7%。与BPH和HC受试者相比，PC中MIC-1的表达增加与PC报告中MIC-1的过度表达同时发生，并证实了其他研究的矛盾结果。
To reduce the ambiguity of contradictory observations in different studies regarding the expression level of Macrophage Inhibitory Cytokine-1 (MIC-1) in serum in prostate cancer (PC), benign prostatic hyperplasia (BPH) and healthy controls (HC), we designed this double-blind study. The study comprises 240 sera from PC, BPH and HC subjects. The expression level of MIC-1 in PC, BPH and HC were appraised using Western blot (WB) and ELISA based approach. WB and ELISA appraisal reveals that the expression level of MIC-1 is significantly higher in PC than in HC or BPH subjects. Regression analysis revealed a significant correlation between MIC-1 vs. PSA (r = 0.09; p < 0.001) and MIC-1 vs. GS (r = 0.7; p < 0.001). ROC analysis using discriminant predicted probability revealed that the MIC-1 was better than PSA. Moreover, the combination of MIC-1 and PSA was allowing 99.1% AUC for the differentiation of BPH + PC from HC, 97.9% AUC for differentiation of BPH from HC, 98.6% AUC for differentiation of PC from HC, and 96.7% AUC for the differentiation of PC from BPH. The augmented expression of MIC-1 in PC compared to BPH and HC subjects is in concurrent of the over-expression of MIC-1 in PC reports and confiscates the contradictory findings of other studies.
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