The clinicopathological significance of REIC expression in colorectal carcinomas.
The clinicopathological significance of REIC expression in colorectal carcinomas.
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REIC在结直肠癌中表达的临床病理意义。
DOI：
10.14670/hh-27.735
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发表时间：
2012-06
影响因子：
2
通讯作者：
Zheng, H-C
中科院分区：
文献类型：
--
作者：
Zhu, W;Xu, X-Y;Nie, X-C;Yang, X;Xing, Y-N;Yu, M;Liu, Y-P;Takano, Y;Zheng, H-C
REIC在永生化细胞系中表达下调，并能抑制集落形成、肿瘤生长和诱导凋亡。在这里，它的表达进行了检查，免疫组织化学组织芯片包含结直肠非肿瘤性粘膜（NNM），腺瘤和腺癌。采用Western blot、RT-PCR和酶联免疫吸附试验（ELISA）检测大肠癌组织和细胞系中REIC的表达及其分泌水平。结果表明，REIC在Colo 201、Colo 205、DLD-1、HCT-15、HCT-116、HT-29、KM-12、SW 480、SW 620和WiDr中差异表达，其分泌浓度小于300 pg/mL。癌组织的REIC表达低于匹配的NNM，但蛋白质含量无差异。免疫组化结果显示，REIC的表达从NNM、腺瘤到腺癌明显降低（p<0.05）。REIC表达与肿瘤浸润深度、TNM分期、去分化程度、Capase-3和核生长抑制因子5（ING 5）表达呈负相关（p<0.05），而与年龄、性别、肿瘤大小、淋巴结或静脉浸润、淋巴结转移无关（p>0.05）。Kaplan-Meier分析显示REIC表达与大肠癌的预后无关（p>0.05）。考克斯分析显示，淋巴结和静脉侵犯、淋巴结转移和UICC分期是胃癌的独立预后因素（p<0.05）。我们的研究表明，REIC表达下调可能在大肠腺瘤-腺癌序列和随后的进展中起重要作用。REIC异常表达可作为大肠癌发生发展的一个良好指标。
REIC is down-regulated in immortalized cell lines compared with the parental normal counterparts, and could inhibit colony formation, tumor growth and induce apoptosis. Here, its expression was examined by immunohistochemistry on tissue microarray containing colorectal non-neoplastic mucosa (NNM), adenoma and adenocarcinoma. Colorectal carcinoma tissue and cell lines were studied for REIC expression or its secretory level by Western blot, RT-PCR or enzyme-linked immunosorbent assay (ELISA). The results demonstrated that REIC was differentially expressed in Colo201, Colo205, DLD-1, HCT-15, HCT-116, HT-29, KM-12, SW480, SW620, and WiDr with its secretion concentration less than 300 pg/mL. Carcinomas showed statistically lower REIC expression than matched NNM with no difference for protein content. Immunohistochemically, REIC expression was significantly decreased from NNM, adenoma to adenocarcinoma (p<0.05). REIC expression was negatively correlated with depth of invasion, TNM staging, dedifferentiation, Capase-3 and nuclear inhibitor of growth 5 (ING5) expression (p<0.05), while not with age, sex, tumor size, lymphatic or venous invasion, or lymph node metastasis (p>0.05). Kaplan-Meier analysis indicated that REIC expression was not associated with the prognosis of colorectal carcinomas (p>0.05). Cox's analysis demonstrated that lymphatic and venous invasion, lymph node metastasis, and UICC staging were independent prognostic factors for carcinoma (p<0.05). Our study indicated that down-regulated REIC expression might play an important role in colorectal adenoma-adenocarcinoma sequence and subsequent progression. Aberrant REIC expression might be employed as a good marker of pathogenesis and development of colorectal carcinomas.
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DOI：
10.1016/s0022-5347(18)31309-0
发表时间：
2007-04
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
S. Urakami;H. Shiina;H. Enokida;Toshifumi Kawakami;K. Kawamoto;H. Hirata;Yuichiro Tanaka;N. Kikuno;M. Nakagawa;M. Igawa;R. Dahiya
通讯作者：
S. Urakami;H. Shiina;H. Enokida;Toshifumi Kawakami;K. Kawamoto;H. Hirata;Yuichiro Tanaka;N. Kikuno;M. Nakagawa;M. Igawa;R. Dahiya
影响因子：
3.8
作者：
Veeck J;Wild PJ;Fuchs T;Schüffler PJ;Hartmann A;Knüchel R;Dahl E
通讯作者：
Dahl E
影响因子：
4.3
作者：
Ding, Zhen;Qian, Ye-Ben;Xiong, Qi-Ru
通讯作者：
Xiong, Qi-Ru
影响因子：
4.3
作者：
Bin Yang;Z. Du;Yingtang Gao;Cheng Lou;Shi-guang Zhang;T. Bai;Yi-jun Wang;Wen-qin Song
通讯作者：
Bin Yang;Z. Du;Yingtang Gao;Cheng Lou;Shi-guang Zhang;T. Bai;Yi-jun Wang;Wen-qin Song
影响因子：
6.4
作者：
Lee, Eun-Ju;Jo, Minwha;Lee, Je-Ho
通讯作者：
Lee, Je-Ho