Surface display of Aggregatibacter actinomycetemcomitans autotransporter Aae and dispersin B hybrid act as antibiofilm agents.
Surface display of Aggregatibacter actinomycetemcomitans autotransporter Aae and dispersin B hybrid act as antibiofilm agents.
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放线聚集菌自转运蛋白 Aae 和分散素 B 杂交体的表面展示充当抗生物膜剂。
DOI：
10.1111/omi.12126
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发表时间：
2016
影响因子：
3.7
通讯作者：
Ramasubbu,N
中科院分区：
文献类型：
--
作者：
Ragunath,C;DiFranco,K;Shanmugam,M;Gopal,P;Vyas,V;Fine,DH;Cugini,C;Ramasubbu,N
Among the various proteins expressed by the periodontopathogenAggregatibacter actinomycetemcomitans, two proteins play important roles for survival in the oral cavity. The autotransporter Aae facilitates the attachment of the pathogen to oral epithelial cells, which act as a reservoir, while the biofilm‐degrading glycoside hydrolase dispersin B facilitates the movement of daughter cells from the mature biofilm to a new site. The objective of this study was to use the potential of these two proteins to control biofilms. To this end, we generated a hybrid construct between the Aae C‐terminal translocating domain and dispersin B, and mobilized it intoEscherichia coliRosetta (DE3) pLysS cells. Immunofluorescence analysis of the modifiedE. colicells confirmed the presence of dispersin B on the surface. Further, the membrane localization of the displayed dispersin B was confirmed with Western blot analysis. The integrity of theE. colicells displaying the dispersin B was confirmed through FACS analysis. The hydrolytic activity of the surface‐displayed dispersin B was confirmed by using 4‐methylumbelliferyl‐β‐d‐glucopyranoside as the substrate. The detachment ability of the dispersin B surface‐displayingE. colicells was shown usingStaphylococcus epidermidisandActinobacillus pleuropneumoniaebiofilms in a microtiter assay. We concluded that the Aae β‐domain is sufficient to translocate foreign enzymes in the native folded form and that the method of Aae‐mediated translocation of surface displayed enzymes might be useful for control of biofilms.
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影响因子：
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作者：
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DOI：
--
发表时间：
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期刊：
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DOI：
--
发表时间：
2012
期刊：
影响因子：
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N. Ramasubbu
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影响因子：
3.8
作者：
A. Freeman;S. Abramov;G. Georgiou
通讯作者：
G. Georgiou