Nanoscale surface modification favors benign biofilm formation and impedes adherence by pathogens.
Nanoscale surface modification favors benign biofilm formation and impedes adherence by pathogens.
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DOI：
10.1016/j.nano.2011.11.014
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发表时间：
2012-04
影响因子：
5.4
通讯作者：
Cai, Chengzhi
中科院分区：
文献类型：
--
作者：
Trautner, Barbara W.;Lopez, Analette I.;Kumar, Amit;Siddiq, Danish M.;Liao, Kershena S.;Li, Yan;Tweardy, David J.;Cai, Chengzhi
We have found in vitro that a biofilm of benign Escherichia coli 83972 interferes with urinary catheter colonization by pathogens, and in human studies E. coli 83972-coated urinary catheters are associated with lower rates of catheter-associated urinary tract infections. We hypothesized that modifying surfaces to present mannose ligands for the type 1 fimbriae of E. coli would promote formation of dense E. coli 83972 biofilms, thereby interfering with surface colonizaion by Enterococcus faecalis, a common uropathogen. We covalently immobilized mannose on silicon substrates by attaching amino-terminated mannose derivative to carboxylic acid-terminated monolayers via amidation. Fluorescence microscopy showed that E. coli 83972 adherence to mannose-modified surfaces increased 4.4-fold compared to unmodified silicon surfaces. Pre-exposing mannose-modified surfaces to E. coli 83972 established a protective biofilm that reduced E. faecalis adherence by 83-fold. Mannose-fimbrial interactions were essential for the improved E. coli 83927 adherence and interference effects.
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影响因子：
3.9
作者：
Wang, H;Chen, SF;Jiang, SY
通讯作者：
Jiang, SY
影响因子：
3.9
作者：
Cicero, RL;Linford, MR;Chidsey, CED
通讯作者：
Chidsey, CED
影响因子：
3.9
作者：
Perring, M;Dutta, S;Bowden, NB
通讯作者：
Bowden, NB
影响因子：
2.1
作者：
Trautner, BW;Hull, RA;Darouiche, RO
通讯作者：
Darouiche, RO
影响因子：
--
作者：
WARREN J W;ANTHONY W C;MUNCIE H L JR
通讯作者：
MUNCIE H L JR