Functional interaction of cockroach allergens and mannose receptor (CD206) in human circulating fibrocytes.
Functional interaction of cockroach allergens and mannose receptor (CD206) in human circulating fibrocytes.
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DOI：
10.1371/journal.pone.0064105
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gao PS
中科院分区：
文献类型：
--
作者：
Tsai YM;Hsu SC;Zhang J;Zhou YF;Plunkett B;Huang SK;Gao PS
先天模式识别C型凝集素受体（CLRs），包括甘露糖受体（MRC 1; CD 206），已被认为与过敏原功能性相互作用，并且在控制免疫应答中是关键的。纤维细胞被认为在过敏性哮喘中起作用。在这里，我们试图研究蟑螂过敏原与CD 206在纤维细胞中的功能相互作用。采用基质辅助激光解吸电离质谱（MALDI-MS）技术对天然纯化的蟑螂变应原Bla g 2的N-连接聚糖进行了分析，并采用固相结合试验测定了蟑螂变应原与CD 206的结合活性。测定人纤维细胞上的CD 206表达水平和Bla g 2处理的纤维细胞中CD 206介导的信号传导和细胞因子产生。对Bla g 2的N-连接聚糖的分析显示，含有和不含岩藻糖的小甘露糖封端聚糖占优势。观察到Bla g 2与CD 206的显著结合，其被酵母甘露聚糖（已知的CD 206配体）、游离甘露糖和阻断抗体（抗hMR）抑制。人纤维细胞（CD 45+和胶原蛋白-1+）的流式细胞术分析显示，纤维细胞上的CD 206的选择性表达。在功能上，观察到纤维细胞对FITC标记的Bla g 2的浓度依赖性摄取，但抗hMR显著抑制。Bla g 2可刺激培养的纤维细胞中炎性细胞因子包括TNF-α和IL-6的上调以及核因子κ B（NF-κ B/p65）、p38丝裂原活化蛋白激酶（p38）、ERK和JNK的活化。这种增加的TNF-α和IL-6的分泌以及NF-κ B、ERK和JNK的活化被添加甘露聚糖或甘露糖显著抑制。此外，Bla g 2诱导的TNF-α和IL-6产生的增加也被NF-κ B、ERK和JNK抑制剂的使用抑制。这些结果提供了证据支持在人类纤维细胞中存在功能性蟑螂变应原-CD 206轴，表明CD 206在调节变应原诱导的哮喘过敏反应中的作用。
The innate pattern recognition C-type-lectin receptors (CLRs), including mannose receptor (MRC1; CD206), have been suggested to functionally interact with allergens and are critical in controlling immune response. Fibrocytes have been considered to play a role in allergic asthma. Here we sought to investigate the functional interaction of cockroach allergens with CD206 in fibrocytes. Profiling of N-linked glycans from natural purified cockroach allergen Bla g 2 was accomplished by MALDI-MS. The binding activity of cockroach allergens to CD206 was determined by solid-phase binding assays. Levels of CD206 expression on human fibrocytes and CD206 mediated signaling and cytokine production in Bla g 2 treated fibrocytes were determined. Profiling of N-linked glycans from Bla g 2 revealed a predominance of small, mannose-terminated glycans with and without fucose. Significant binding of Bla g 2 to CD206 was observed, which was inhibited by yeast mannan (a known CD206 ligand), free mannose, and a blocking antibody (anti-hMR). Flow cytometric analyses of human fibrocytes (CD45+ and collagen-1+) showed selective expression of CD206 on fibrocytes. Functionally, a concentration-dependent uptake of FITC labeled Bla g 2 by fibrocytes was observed, but was significantly inhibited by anti-hMR. Bla g 2 can stimulate up-regulation of inflammatory cytokines including TNF-alpha and IL-6 and activation of nuclear factor kappa B (NF-kB/p65), p38 mitogen-activated protein kinase (p38), ERK, and JNK in cultured fibrocytes. This increased secretion of TNF-alpha and IL-6 and activation of NF-kB, ERK, and JNK was significantly inhibited by the addition of either mannan or mannose. Furthermore, Bla g 2 induced increase in TNF-alpha and IL-6 production was also inhibited by the use of NF-kB, ERK, and JNK inhibitors. These results provide evidence supporting the existence of a functional cockroach allergen-CD206 axis in human fibrocytes, suggesting a role for CD206 in regulating allergen induced allergic responses in asthma.
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影响因子：
4.8
作者：
Hsu, Shih-Chang;Chen, Chien-Ho;Huang, Shau-Ku
通讯作者：
Huang, Shau-Ku
DOI：
10.1073/pnas.94.12.6307
发表时间：
1997-06-10
影响因子：
11.1
作者：
Chesney, J;Bacher, M;Bucala, R
通讯作者：
Bucala, R
影响因子：
14.2
作者：
Gruchalla, RS;Pongracic, J;Mitchell, H
通讯作者：
Mitchell, H
DOI：
10.1111/j.1365-2222.2010.03561.x
发表时间：
2010-09
期刊：
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
影响因子：
--
作者：
Gao P;Grigoryev DN;Rafaels NM;Mu D;Wright JM;Cheadle C;Togias A;Beaty TH;Mathias RA;Schroeder JT;Barnes KC
通讯作者：
Barnes KC
影响因子：
9
作者：
Kao, Huang-Kai;Chen, Bin;Guo, Lifei
通讯作者：
Guo, Lifei