Using root DNA to assess responses to phosphorus by surface roots in wheat and barley
Using root DNA to assess responses to phosphorus by surface roots in wheat and barley
复制标题
使用根 DNA 评估小麦和大麦表面根对磷的反应
作者：
G. McDonald;A. Mckay;C. Huang;B. Bovill
背景和目的农业土壤中的大部分磷(P)存在于表层，表土觅食通常被认为是磷回收的重要因素。本研究旨在研究土壤表层根系数量和分布对小麦和大麦生长及磷肥效应的影响。方法在两年多的时间里，对10个品种和20个品种的小麦和大麦在磷敏感部位分别以0和30 kg/hm2的磷进行种植。用Zadok‘s Growth Stage(ZGS)30-31的土壤中根DNA浓度来估算播行间和行内土壤表层10 cm的根数及其比率。结果施磷量增加，根系DNA密度增加，苗行DNA密度增加。根DNA密度在不同品种间存在显著差异，并在不同地点表现出一定的一致性。大麦和小麦的根系侧向伸展程度没有差异，但大麦的行内根系生长对磷的响应大于小麦。生物量、产量和产量对磷的反应与根DNA密度或根DNA在行内和行内的比率没有一致的相关性。结论土壤中根DNA密度和根在行内和行间的分布与磷吸收、产量或对磷的反应没有一致的相关性。
Background and aimsMost phosphorus (P) in agricultural soils is found in the surface layers and topsoil foraging is often considered to be important for P recovery. The aim of this work was to examine how the amount and distribution of roots in the surface soil layer affects growth and response to P fertiliser in wheat and barley.MethodBetween 10 and 20 varieties of wheat and barley were grown at 0 kg P/ha and 30 kg P/ha at P responsive sites over two years. The amount of roots in the surface 10 cm of soil between and within sowing rows and their ratio were assessed using the concentration of root DNA in the soil at Zadok’s growth stage (ZGS) 30–31. Shoot biomass and P concentration were measured at the time of root sampling and plots were harvested for yield.ResultsAdding P increased root DNA density with the greater response observed in the seeding row. Significant differences in root DNA density were measured among varieties and these showed some consistency over sites. The degree of lateral spread of roots did not differ between barley and wheat, but within-row root growth of barley showed a greater response to P than that of wheat. Biomass production, yield and yield response to P were not related consistently to root DNA density nor to the ratio of root DNA between and within rows.ConclusionGenetic differences in root growth in the top 10 cm can be identified but the root DNA density in the soil and the distribution of roots within and between rows were not consistently associated with P uptake, yield or yield response to P.
影响因子：
7.3
作者：
Heppell, J.;Talboys, P.;Roose, T.
通讯作者：
Roose, T.