Regulatory Mechanism of ABA and ABI3 on Vegetative Development in the Moss Physcomitrella patens.
Regulatory Mechanism of ABA and ABI3 on Vegetative Development in the Moss Physcomitrella patens.
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ABA 和 ABI3 对小立碗藓营养发育的调控机制。
DOI：
10.3390/ijms19092728
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发表时间：
2018-09-12
影响因子：
5.6
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Zhao M;Li Q;Chen Z;Lv Q;Bao F;Wang X;He Y
小立碗藓是研究植物发育过程的模式系统。脱落酸不敏感蛋白3（ABI 3）是阿坝信号通路中的一个转录因子，在维管植物的生长发育中起着重要的作用。为了了解阿坝和PPABI 3对小立碗藓营养生长的调控机制，我们对野生型（WT）植物和小立碗藓3突变体进行了生理、细胞和RNA-seq分析。在阿坝处理过程中，配子体的生长受到抑制的程度较低，与WT植物相比，野生型植物。显微镜观察表明，与WT植株相比，阿坝处理促进了茎丝体和绿丝体的分化。在正常条件下，拟南芥3号植株中生长素含量显著高于野生型植株。生长素诱导茎丝体从绿丝体分化为茎丝体，并且拟南芥3植株的表型与外源吲哚-3-乙酸（IAA）处理的WT植株相似。RNA-seq分析表明，PpABI 3调控的基因与阿坝调控的基因有重叠，阿坝调控的生长素相关基因中约78%与PpABI 3调控的基因有重叠。这些结果表明，阿坝对展枝海棠营养生长的影响部分是通过PpABI 3的调控实现的; PpABI 3是展枝海棠营养生长的负调控因子，阿坝和PpABI 3对展枝海棠营养生长的调控可能是通过调控生长素相关基因的表达实现的。PPABI 3可能与展叶海棠中阿坝和生长素之间的交互作用有关。
The moss Physcomitrella patens is a model system for studying plant developmental processes. ABSCISIC ACID INSENSITIVE3 (ABI3), a transcription factor of the ABA signaling pathway, plays an important role in plant growth and development in vascular plant. To understand the regulatory mechanism of ABA and PpABI3 on vegetative development in Physcomitrella patens, we applied physiological, cellular, and RNA-seq analyses in wild type (WT) plants and ∆abi3 mutants. During ABA treatment, the growth of gametophytes was inhibited to a lesser extent ∆abi3 plants compared with WT plants. Microscopic observation indicated that the differentiation of caulonemata from chloronemata was accelerated in ∆abi3 plants when compared with WT plants, with or without 10 μM of ABA treatment. Under normal conditions, auxin concentration in ∆abi3 plants was markedly higher than that in WT plants. The auxin induced later differentiation of caulonemata from chloronemata, and the phenotype of ∆abi3 plants was similar to that of WT plants treated with exogenous indole-3-acetic acid (IAA). RNA-seq analysis showed that the PpABI3-regulated genes overlapped with genes regulated by the ABA treatment, and about 78% of auxin-related genes regulated by the ABA treatment overlapped with those regulated by PpABI3. These results suggested that ABA affected vegetative development partly through PpABI3 regulation in P. patens; PpABI3 is a negative regulator of vegetative development in P. patens, and the vegetative development regulation by ABA and PpABI3 might occur by regulating the expression of auxin-related genes. PpABI3 might be associated with cross-talk between ABA and auxin in P. patens.
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DOI：
10.1073/pnas.92.8.3483
发表时间：
1995-04-11
影响因子：
11.1
作者：
BALLAS, N;WONG, LM;THEOLOGIS, A
通讯作者：
THEOLOGIS, A
DOI：
10.1139/b93-183
发表时间：
1993-11-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
GOODE, JA;STEAD, AD;DUCKETT, JG
通讯作者：
DUCKETT, JG
影响因子：
7.4
作者：
Horstman, Anneke;Li, Mengfan;Boutilier, Kim
通讯作者：
Boutilier, Kim
影响因子：
11.6
作者：
Belin, Christophe;Megies, Christian;Lopez-Molina, Luis
通讯作者：
Lopez-Molina, Luis
影响因子：
11.4
作者：
Marchant, A;Kargul, J;Bennett, MJ
通讯作者：
Bennett, MJ