Stability of influenza vaccine coated onto microneedles.
Stability of influenza vaccine coated onto microneedles.
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DOI：
10.1016/j.biomaterials.2012.01.054
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发表时间：
2012-05
期刊：
影响因子：
14
通讯作者：
Prausnitz, Mark R.
中科院分区：
文献类型：
--
作者：
Choi, Hyo-Jick;Yoo, Dae-Goon;Bondy, Brian J.;Quan, Fu-Shi;Compans, Richard W.;Kang, Sang-Moo;Prausnitz, Mark R.
关键词：
涂有疫苗的微针贴片通过使用基于贴片的递送方法简化了疫苗接种，并将疫苗靶向到皮肤上，与肌肉注射相比具有更好的免疫原性。先前对微针的研究已经证明使用新鲜制备的微针进行有效的疫苗接种，但是疫苗的长期稳定性问题只得到有限的关注。本研究假设微针包被基质的结晶和相分离破坏了包被在微针上的流感疫苗，并对整个灭活疫苗包被微针的长期稳定性进行了研究。体外实验表明，以血凝活性衡量，疫苗的稳定性与包被基质结晶和相分离程度成正比。透射电镜同样显示与结晶相关的灭活病毒疫苗的破坏形态。在小鼠体内对免疫反应和保护效果的评估进一步表明，使用结晶或相分离涂层的微针接种流感疫苗后，疫苗的免疫原性降低。研究表明，干燥包衣基质的结晶和相分离是影响流感疫苗包衣微针长期稳定性的重要因素。
A microneedle patch coated with vaccine simplifies vaccination by using a patch-based delivery method and targets vaccination to the skin for superior immunogenicity compared to intramuscular injection. Previous studies of microneedles have demonstrated effective vaccination using freshly prepared microneedles, but the issue of long-term vaccine stability has received only limited attention. Here, we studied the long-term stability of microneedles coated with whole inactivated influenza vaccine guided by the hypothesis that crystallization and phase separation of the microneedle coating matrix damages influenza vaccine coated onto microneedles. In vitro showed that the vaccine lost stability as measured by hemagglutination activity in proportion to the degree of coating matrix crystallization and phase separation. Transmission electron microscopy similarly showed damaged morphology of the inactivated virus vaccine associated with crystallization. In vivo assessment of immune response and protective efficacy in mice further showed reduced vaccine immunogenicity after influenza vaccination using microneedles with crystallized or phase-separated coatings. This work shows that crystallization and phase separation of the dried coating matrix are important factors affecting long-term stability of influenza vaccine-coated microneedles.
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DOI：
10.1086/649228
发表时间：
2010-01-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Kim YC;Quan FS;Yoo DG;Compans RW;Kang SM;Prausnitz MR
通讯作者：
Prausnitz MR
影响因子：
3.7
作者：
Daddona, Peter E.;Matriano, James. A.;Maa, Yuh-Fun
通讯作者：
Maa, Yuh-Fun
影响因子：
3.7
作者：
Fernando GJ;Chen X;Prow TW;Crichton ML;Fairmaid EJ;Roberts MS;Frazer IH;Brown LE;Kendall MA
通讯作者：
Kendall MA
影响因子：
3.7
作者：
Corbett HJ;Fernando GJ;Chen X;Frazer IH;Kendall MA
通讯作者：
Kendall MA
影响因子：
6.4
作者：
Koutsonanos, Dimitrios G.;Martin, Maria del Pilar;Skountzou, Ioanna
通讯作者：
Skountzou, Ioanna