Enhanced memory responses to seasonal H1N1 influenza vaccination of the skin with the use of vaccine-coated microneedles.
Enhanced memory responses to seasonal H1N1 influenza vaccination of the skin with the use of vaccine-coated microneedles.
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DOI：
10.1086/649228
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发表时间：
2010-01-15
期刊：
影响因子：
--
通讯作者：
Prausnitz MR
中科院分区：
文献类型：
--
作者：
Kim YC;Quan FS;Yoo DG;Compans RW;Kang SM;Prausnitz MR
流感的发病率和死亡率可以通过改进疫苗接种来降低。为了开发一种新的皮肤给药方法，使其简单和自我管理，我们制备了微针贴剂与稳定的流感疫苗，并研究其保护性免疫反应。用单剂量海藻糖稳定的流感疫苗免疫的小鼠产生了强烈的抗体应答，这些抗体应答是长期存在的。与传统的肌内免疫相比，稳定的微针疫苗接种在诱导保护性免疫方面具有上级优势，如在致死攻击后有效的肺病毒清除和增强的体液和抗体分泌细胞免疫应答所证明的。发现疫苗稳定性是重要的，因为用不稳定的微针疫苗免疫的小鼠引起较弱的IgG2a抗体应答，并且仅部分保护免受病毒攻击。通过微针递送到皮肤来改善树突状细胞向局部淋巴结的运输可能在促进保护性免疫方面发挥作用。这些发现表明，使用微针贴片在皮肤中接种疫苗可以提高保护效力，诱导长期持续的免疫原性，并可能提供一种简单的给药方法来改善流感疫苗接种。
Morbidity and mortality due to influenza could be reduced by improved vaccination. To develop a novel skin delivery method for simple and self administration, we prepared microneedle patches with stabilized influenza vaccine and investigated their protective immune responses. Mice immunized by a single microneedle dose of trehalose-stabilized influenza vaccine developed strong antibody responses that were long-lived. Compared to traditional intramuscular immunization, stabilized microneedle vaccination was superior in inducing protective immunity as evidenced by efficient lung viral clearance and enhanced humoral and antibody secreting cell immune responses after lethal challenge. Vaccine stabilization was found to be important, because mice immunized with an unstabilized microneedle vaccine elicited weaker IgG2a antibody response and were only partially protected against viral challenge. Improved trafficking of dendritic cells to regional lymph nodes by microneedle delivery to the skin might play a role in contributing to improved protective immunity. These findings suggest that vaccination in the skin using a microneedle patch can improve protective efficacy, induce long-term sustained immunogenicity, and may provide a simple method of administration to improve influenza vaccination.
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影响因子：
5.4
作者：
Quan, Fu-Shi;Compans, Richard W.;Kang, Sang-Moo
通讯作者：
Kang, Sang-Moo
DOI：
10.1038/nri1310
发表时间：
2004-03
期刊：
Nature reviews. Immunology
影响因子：
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作者：
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6.5
作者：
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通讯作者：
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影响因子：
158.5
作者：
Belshe, RB;Newman, FK;Dubin, G
通讯作者：
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