Transcription of the cottontail rabbit papillomavirus early region and identification of two E6 polypeptides in COS-7 cells
Transcription of the cottontail rabbit papillomavirus early region and identification of two E6 polypeptides in COS-7 cells
复制标题
棉尾兔乳头瘤病毒早期区在COS-7细胞中的转录及两条E6多肽的鉴定
作者：
M. Barbosa;F. Wettstein
棉尾兔乳头瘤病毒（CRPV）早期蛋白在病毒诱导的肿瘤中以非常低的水平存在，并且不能通过免疫学方法检测到。此外，培养物中的细胞不容易被病毒转化。为了克服鉴定和表征由E6开放阅读框编码的推定转化蛋白的这些困难，在晚期猿猴病毒40启动子的控制下表达早期棉尾兔乳头状瘤病毒区域。在瞬时转染COS-7细胞中的转录物的映射表明，转录开始在猴病毒40的晚期区域。鉴定了两种E6编码的多肽，代表在第一和第二AUG密码子处起始的翻译产物。
Cottontail rabbit papillomavirus (CRPV) early proteins are present at very low levels in virus-induced tumors and cannot be detected by immunological methods. Furthermore, cells in culture are not readily transformed by the virus. To overcome these difficulties in identifying and characterizing the putative transforming protein(s) coded by the E6 open reading frame, the early cottontail rabbit papillomavirus region was expressed under the control of the late simian virus 40 promoter. Mapping of the transcripts in transiently transfected COS-7 cells indicated that transcription was initiated in the late region of simian virus 40. Two E6-coded polypeptides were identified, representing translation products initiated at the first and second AUG codons.
DOI：
10.1073/pnas.83.13.4680
发表时间：
1986-07-01
影响因子：
11.1
作者：
SMOTKIN, D;WETTSTEIN, FO
通讯作者：
WETTSTEIN, FO
宫颈腺癌和腺鳞癌中的人乳头瘤病毒脱氧核糖核酸。
影响因子：
7.2
作者：
Smotkin,D;Berek,JS;Fu,YS;Hacker,NF;Major,FJ;Wettstein,FO
通讯作者：
Wettstein,FO