The Cpx envelope stress response both facilitates and inhibits elaboration of the enteropathogenic Escherichia coli bundle-forming pilus.
The Cpx envelope stress response both facilitates and inhibits elaboration of the enteropathogenic Escherichia coli bundle-forming pilus.
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DOI：
10.1111/j.1365-2958.2010.07145.x
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发表时间：
2010-06-01
影响因子：
3.6
通讯作者：
Raivio TL
中科院分区：
文献类型：
--
作者：
Vogt SL;Nevesinjac AZ;Humphries RM;Donnenberg MS;Armstrong GD;Raivio TL
Cpx包膜应激反应由周质蛋白的错误折叠诱导，并通过上调几种周质蛋白折叠和降解因子来恢复包膜稳态。Cpx反应还调节多种跨鞭毛蛋白复合物的表达，包括鞭毛、分泌系统和皮利，其在发病机制中起重要作用。在先前的研究中，我们灭活了肠致病性大肠杆菌（EPEC）中的Cpx反应，EPEC是婴儿腹泻的病原体，并观察到其主要粘附素的表达减少，形成菌毛的菌毛（BFP）。在这里，我们研究了这种BFP表达缺陷的机制，发现这种表型可以归因于周质折叠因子，如DsbA，DegP和CpxP的表达不足。因此，低水平的Cpx途径活性通过上调对BFP组分蛋白的折叠重要的因子来促进BFP合成。相反，我们发现，完全诱导的Cpx反应抑制BFP的表达，主要是通过抑制转录的bfp基因簇。结合以前的报告检查EPEC III型分泌，我们的研究结果表明，Cpx响应协调的抑制细胞表面结构在信封应力期间。
The Cpx envelope stress response is induced by the misfolding of periplasmic proteins and restores envelope homeostasis by upregulating several periplasmic protein folding and degrading factors. The Cpx response also regulates the expression of a variety of envelope-spanning protein complexes, including flagella, secretion systems and pili, which play an important role in pathogenesis. In a previous study, we inactivated the Cpx response in enteropathogenic Escherichia coli (EPEC), a causative agent of infant diarrhoea, and observed decreased expression of its major adhesin, the bundle-forming pilus (BFP). Here, we examined the mechanism underlying this BFP expression defect, and found that this phenotype can be attributed to insufficient expression of periplasmic folding factors, such as DsbA, DegP and CpxP. Hence, a low level of Cpx pathway activity promotes BFP synthesis by upregulating factors important for folding of BFP component proteins. Conversely, we found that full induction of the Cpx response inhibits BFP expression, mainly by repressing transcription of the bfp gene cluster. In combination with a previous report examining EPEC type III secretion, our results demonstrate that the Cpx response co-ordinates the repression of cell-surface structures during periods of envelope stress.
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影响因子：
3.2
作者：
Anantha, TP;Stone, KD;Donnenberg, MS
通讯作者：
Donnenberg, MS
影响因子：
3.1
作者：
Anantha, RP;Stone, KD;Donnenberg, MS
通讯作者：
Donnenberg, MS
影响因子：
2.8
作者：
Daniel, Anu;Singh, Aparna;Donnenberg, Michael S.
通讯作者：
Donnenberg, Michael S.
影响因子：
10.5
作者：
DANESE, PN;SNYDER, WB;SILHAVY, TJ
通讯作者：
SILHAVY, TJ
影响因子：
3.2
作者：
De Wulf, P;Kwon, O;Lin, ECC
通讯作者：
Lin, ECC