Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin.
Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin.
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DOI：
10.1038/jid.2009.243
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发表时间：
2010-01
影响因子：
6.5
通讯作者：
Gallo, Richard L.
中科院分区：
文献类型：
--
作者：
Cogen, Anna L.;Yamasaki, Kenshi;Sanchez, Katheryn M.;Dorschner, Robert A.;Lai, Yuping;MacLeod, Daniel T.;Torpey, Justin W.;Otto, Michael;Nizet, Victor;Kim, Judy E.;Gallo, Richard L.
抗微生物肽是抵抗细菌和病毒等入侵生物的第一道先天免疫防御。在这项研究中，我们假设由人类皮肤的正常微生物居民，表皮葡萄球菌，产生的肽也可能作为一个抗菌盾牌，并有助于表皮界面的正常防御。通过圆二色谱和色氨酸光谱分析表明，S.与哺乳动物AMP如LL-37类似，表皮细胞具有α-螺旋特征和强的脂质膜相互作用。这两种PSM直接诱导脂质囊泡渗漏，并对皮肤病原体如金黄色葡萄球菌发挥选择性抗菌作用。PSM在功能上相互合作并与LL-37合作以增强抗菌作用。此外，PSM减少了A组链球菌（GAS），但没有S的存活。epidermidis在小鼠皮肤上。因此，这些数据表明，PSMγ和PSMδ的产生由S.表皮炎可以通过选择性地抑制皮肤病原体的存活同时维持正常的皮肤微生物组而有益于皮肤免疫防御。
Antimicrobial peptides serve as a first line of innate immune defense against invading organisms such as bacteria and viruses. In this study, we hypothesized that peptides produced by a normal microbial resident of human skin, Staphylococcus epidermidis, might also act as an antimicrobial shield and contribute to normal defense at the epidermal interface. We show by circular dichroism and tryptophan spectroscopy that phenol-soluble modulins (PSMs) γ and δ produced by S. epidermidis have an α-helical character and a strong lipid membrane interaction similar to mammalian AMPs such as LL-37. Both PSMs directly induced lipid vesicle leakage and exerted selective antimicrobial action against skin pathogens such as Staphylococcus aureus. PSMs functionally cooperated with each other and LL-37 to enhance antimicrobial action. Moreover, PSMs reduced Group A Streptococcus (GAS) but not the survival of S. epidermidis on mouse skin. Thus, these data suggest that the production of PSMγ and PSMδ by S. epidermidis can benefit cutaneous immune defense by selectively inhibiting the survival of skin pathogens while maintaining the normal skin microbiome.
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DOI：
10.1093/protein/8.3.315
发表时间：
1995-03-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
DHOPLE, VM;NAGARAJ, R
通讯作者：
NAGARAJ, R
影响因子：
64.8
作者：
Nizet, V;Ohtake, T;Gallo, RL
通讯作者：
Gallo, RL
影响因子：
4.8
作者：
Dorschner, RA;Lopez-Garcia, B;Gallo, RL
通讯作者：
Gallo, RL
影响因子：
2.9
作者：
Porcelli, Fernando;Verardi, Raffaello;Veglia, Gianluigi
通讯作者：
Veglia, Gianluigi
影响因子：
4.9
作者：
HALE, EM;HINSDILL, RD
通讯作者：
HINSDILL, RD