Metaproteogenomic Profiling of Microbial Communities Colonizing Actively Venting Hydrothermal Chimneys.
Metaproteogenomic Profiling of Microbial Communities Colonizing Actively Venting Hydrothermal Chimneys.
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DOI：
10.3389/fmicb.2018.00680
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发表时间：
2018
影响因子：
5.2
通讯作者：
Meyerdierks A
中科院分区：
文献类型：
--
作者：
Pjevac P;Meier DV;Markert S;Hentschker C;Schweder T;Becher D;Gruber-Vodicka HR;Richter M;Bach W;Amann R;Meyerdierks A
在热液喷口处，还原热液流体与含氧海水接触后形成由硫化物、硫酸盐和氧化物组成的烟囱。这些烟囱的壁和表面是喷口相关微生物的重要栖息地。我们使用社区蛋白质基因组学研究和比较的组成，代谢潜力和相对原位蛋白质丰度的微生物群落殖民两个积极发泄热液烟囱马努斯盆地弧后扩张中心（巴布亚新几内亚）。我们确定了重叠的两个烟囱在原位功能配置文件，尽管微生物群落组成和通风制度的差异。两个烟囱上的碳固定似乎主要是通过反向三羧酸循环介导的，并由硫氧化提供燃料，而通过Calvin-Benson-Bassham循环的氢氧化和碳固定的丰富代谢潜力几乎没有被利用。值得注意的是，高度多样化的微生物群落殖民分析的黑烟囱具有高度冗余的代谢潜力。相比之下，相当少的多样性社区殖民扩散通风烟囱表现出较高的代谢功能。因此，系统发育水平上的多样性增加与定居在热液烟囱中的微生物群落的代谢多样性增加没有直接联系。
At hydrothermal vent sites, chimneys consisting of sulfides, sulfates, and oxides are formed upon contact of reduced hydrothermal fluids with oxygenated seawater. The walls and surfaces of these chimneys are an important habitat for vent-associated microorganisms. We used community proteogenomics to investigate and compare the composition, metabolic potential and relative in situ protein abundance of microbial communities colonizing two actively venting hydrothermal chimneys from the Manus Basin back-arc spreading center (Papua New Guinea). We identified overlaps in the in situ functional profiles of both chimneys, despite differences in microbial community composition and venting regime. Carbon fixation on both chimneys seems to have been primarily mediated through the reverse tricarboxylic acid cycle and fueled by sulfur-oxidation, while the abundant metabolic potential for hydrogen oxidation and carbon fixation via the Calvin–Benson–Bassham cycle was hardly utilized. Notably, the highly diverse microbial community colonizing the analyzed black smoker chimney had a highly redundant metabolic potential. In contrast, the considerably less diverse community colonizing the diffusely venting chimney displayed a higher metabolic versatility. An increased diversity on the phylogenetic level is thus not directly linked to an increased metabolic diversity in microbial communities that colonize hydrothermal chimneys.
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影响因子：
6.4
作者：
Cao H;Wang Y;Lee OO;Zeng X;Shao Z;Qian PY
通讯作者：
Qian PY
影响因子：
3.4
作者：
Ferrera, Isabel;Banta, Amy B.;Reysenbach, Anna-Louise
通讯作者：
Reysenbach, Anna-Louise
影响因子：
4.4
作者：
Huber, JA;Butterfield, DA;Baross, JA
通讯作者：
Baross, JA
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A
影响因子：
5.2
作者：
Brazelton WJ;Nelson B;Schrenk MO
通讯作者：
Schrenk MO