Macrophage phenotype in response to ECM bioscaffolds.
Macrophage phenotype in response to ECM bioscaffolds.
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DOI：
10.1016/j.smim.2017.04.004
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发表时间：
2017-03
影响因子：
7.8
通讯作者：
Badylak SF
中科院分区：
文献类型：
--
作者：
Huleihel L;Dziki JL;Bartolacci JG;Rausch T;Scarritt ME;Cramer MC;Vorobyov T;LoPresti ST;Swineheart IT;White LJ;Brown BN;Badylak SF
巨噬细胞的存在和表型是损伤后愈合反应的关键决定因素。促炎巨噬细胞表型的下调与细胞外基质(ECM)组成的生物支架的治疗有关，但巨噬细胞的表型特征通常局限于少量的非特异性细胞表面标记或表达的蛋白质。本研究测定了原代小鼠骨髓来源巨噬细胞(BMDM)和转化的人单核细胞系(THP-1细胞)对两种不同的常用ECM生物支架的降解产物的反应：膀胱基质(UBM-ECM)和小肠粘膜下层(SIS-ECM)。量化的细胞反应包括基因表达、蛋白表达、常用的细胞表面标志物和功能分析。结果表明，细胞外基质诱导的表型既不同于经典激活的干扰素γ+脂多糖表型，也不同于交替激活的IL-4表型。此外，BMDM和THP-1巨噬细胞对相同刺激的反应不同，UBM-ECM和SIS-ECM生物支架诱导的表型相似但不同。这项研究的结果不仅表征了具有抗炎特性的MECM表型，而且还表明了在不考虑巨噬细胞的来源和单个细胞标志物的限制的情况下就巨噬细胞介导的事件的作用得出结论的风险和挑战。
Macrophage presence and phenotype are critical determinants of the healing response following injury. Downregulation of the pro-inflammatory macrophage phenotype has been associated with the therapeutic use of bioscaffolds composed of extracellular matrix (ECM), but phenotypic characterization of macrophages has typically been limited to small number of non-specific cell surface markers or expressed proteins. The present study determined the response of both primary murine bone marrow derived macrophages (BMDM) and a transformed human mononuclear cell line (THP-1 cells) to degradation products of two different, commonly used ECM bioscaffolds; urinary bladder matrix (UBM-ECM) and small intestinal submucosa (SIS-ECM). Quantified cell responses included gene expression, protein expression, commonly used cell surface markers, and functional assays. Results showed that the phenotype elicited by ECM exposure (MECM) is distinct from both the classically activated IFNγ + LPS phenotype and the alternatively activated IL-4 phenotype. Furthermore, the BMDM and THP-1 macrophages responded differently to identical stimuli, and UBM-ECM and SIS-ECM bioscaffolds induced similar, yet distinct phenotypic profiles. The results of this study not only characterized an MECM phenotype that has anti-inflammatory traits but also showed the risks and challenges of making conclusions about the role of macrophage mediated events without consideration of the source of macrophages and the limitations of individual cell markers.
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影响因子：
14
作者：
Ariganello, Marianne B.;Simionescu, Dan T.;Labow, Rosalind S.;Lee, J. Michael
通讯作者：
Lee, J. Michael
影响因子：
4.9
作者：
Fangradt, Monique;Hahne, Martin;Buttgereit, Frank
通讯作者：
Buttgereit, Frank
DOI：
10.1073/pnas.1300290110
发表时间：
2013-06-04
影响因子：
11.1
作者：
Godwin, James W.;Pinto, Alexander R.;Rosenthal, Nadia A.
通讯作者：
Rosenthal, Nadia A.
影响因子：
3.2
作者：
Francke, Alexander;Herold, Joerg;Braun-Dullaeus, Ruediger C.
通讯作者：
Braun-Dullaeus, Ruediger C.
影响因子：
4.8
作者：
Date, Dipali;Das, Riku;Mahabeleshwar, Ganapati H.
通讯作者：
Mahabeleshwar, Ganapati H.