Macrophage differentiation and polarization on a decellularized pericardial biomaterial.
Macrophage differentiation and polarization on a decellularized pericardial biomaterial.
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DOI：
10.1016/j.biomaterials.2010.09.004
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发表时间：
2011-01
期刊：
影响因子：
14
通讯作者：
Lee, J. Michael
中科院分区：
文献类型：
--
作者：
Ariganello, Marianne B.;Simionescu, Dan T.;Labow, Rosalind S.;Lee, J. Michael
单核细胞来源的巨噬细胞(MDM)存在于生物材料植入过程中，可以增加、减少或改变与生物材料存在相关的炎症和随后的伤口愈合过程。了解MDM对生物材料的响应对于改进组织工程生物材料的预测和设计具有重要意义。这项研究分析了单核细胞在完整的天然胶原上的直接分化。人单核细胞在去细胞牛心包(DBP)、聚二甲基硅氧烷(PDMS)或聚苯乙烯(TCPS)上分化14d后，所有表面的MDMS均释放出比MMP2高的MMP9和相对较少的MMP1。DBP分化的MDM释放较多的基质金属蛋白酶-2，但酸性磷酸酶活性较低。三种表面的MDMS都释放少量的细胞因子，尽管底物有差异：DBP表面的MDMS释放更多的IL-6、IL-8和MCP-1，但释放更少的IL-10和IL-1ra。这项研究提供的证据表明，脱细胞基质上的MDM可能不会被刺激为活化的炎症表型，支持脱细胞基质用于组织工程的潜力。本研究还表明，分化表面影响MDM表型，因此，巨噬细胞与生物材料相互作用的研究设计应仔细检查所采用的特定巨噬细胞培养方法及其对巨噬细胞表型的影响。
The monocyte-derived macrophage (MDM), present at biomaterial implantations, can increase, decrease or redirect the inflammatory and subsequent wound healing process associated with the presence of a biomaterial. Understanding MDM responses to biomaterials is important for improved prediction and design of biomaterials for tissue engineering. This study analyzed the direct differentiation of monocytes on intact, native collagen. Human monocytes were differentiated on decellularized bovine pericardium (DBP), polydimethylsiloxane (PDMS) or polystyrene (TCPS) for 14 d. MDMs on all surfaces released high amounts of MMP-9 compared to MMP-2 and relatively little MMP-1. MDMs differentiated on DBP released more MMP-2, but less acid phosphatase activity. MDMs on all three surfaces released low amounts of cytokines, although substrate differences were found: MDMs on DBP released higher amounts of IL-6, IL-8, and MCP-1 but lower amounts of IL-10 and IL-1ra. This research provides evidence that MDMs on decellularized matrices may not be stimulated towards an activated, inflammatory phenotype, supporting the potential of decellularized matrices for tissue engineering. This study also demonstrated that the differentiation surface affects MDM phenotype and therefore study design of macrophage interactions with biomaterials should scrutinize the specific macrophage culture method utilized and its effects on macrophage phenotype.
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影响因子：
6.9
作者：
Heino, J
通讯作者：
Heino, J
影响因子：
4.9
作者：
Ariganello, Marianne B.;Labow, Rosalind S.;Lee, J. Michael
通讯作者：
Lee, J. Michael
影响因子：
3.2
作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
Labow, Rosalind S.
影响因子：
5.5
作者：
Lolmede, Karine;Campana, Lara;Rovere-Querini, Patrizia
通讯作者：
Rovere-Querini, Patrizia