The effect of an adding histidine on biological activity and stability of Pc-pis from Pseudosciaena crocea.
The effect of an adding histidine on biological activity and stability of Pc-pis from Pseudosciaena crocea.
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添加组氨酸对大黄鱼Pc-pis生物活性和稳定性的影响。
DOI：
10.1371/journal.pone.0083268
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhong S
中科院分区：
文献类型：
--
作者：
Mao Y;Niu S;Xu X;Wang J;Su Y;Wu Y;Zhong S
Pc-pis 是在大黄鱼中鉴定出的一种新型的 piscidin 样抗菌多肽。尽管 Pc-pis 对大多数测试的细菌都有活性，但对嗜水气单胞菌和铜绿假单胞菌没有活性。 Pc-pis 类似物 Pc-pis-His 是通过在羧基末端添加组氨酸残基而设计的。与 Pc-pis 相比，Pc-pis-His 对一组代表性微生物表现出更广谱和更强的抗菌活性，并且对白点虫营养体具有更有效的抗寄生虫活性。稳定性测定表明，Pc-pis-His 不仅在酸性（pH 5.5-7.3）和相对低浓度的单价阳离子（0-160 mM NaCl）环境中具有抗金黄色葡萄球菌活性，而且在碱性（pH 7.5-9.5）、二价阳离子（1.25-160 mM MgCl2 和 1.25-40 mM CaCl2）和高浓度单价阳离子环境中也具有活性。 (320–2560 mM NaCl) 环境，这表明添加的组氨酸残基赋予 Pc-pis-His 更好的盐、酸和碱耐受性。 Pc-pis-His 还具有所需的耐热性，煮沸 10-60 分钟后肽的抗菌活性反映了这一点。溶血活性分析表明，浓度高达 6 µM 的 Pc-pis-His 对人红细胞没有溶血作用，而 6 µM 的浓度对大多数测试的微生物具有高度活性，尽管与 Pc-pis 相比，Pc-pis-His 的溶血活性有所增强。这些结果为设计具有强效、广谱和稳定抗菌活性的新型鱼藻素提供了独特、合理的基础，并为鱼藻素作为抗微生物和体外原生动物寄生虫感染的潜在治疗剂的未来发展提供了新的见解。
Pc-pis is a novel piscidin-like antimicrobial polypeptide that was identified in Pseudosciaena crocea. Although active against most bacteria tested, Pc-pis was inactive against Aeromonas hydrophila and Pseudomonas aeruginosa. The Pc-pis analogue Pc-pis-His was designed by adding a histidine residue at the carboxyl terminal. Pc-pis-His demonstrated a more broad-spectrum and stronger antimicrobial activity against a representative set of microorganisms and more potent antiparasitic activity against Cryptocaryon irritans trophonts than Pc-pis. The stability assay revealed that Pc-pis-His was active against Staphylococcus aureus not only in acidic (pH 5.5–7.3) and relatively low concentration monovalent cation (0–160 mM NaCl) environments but also in alkaline (pH 7.5–9.5), divalent cation (1.25–160 mM MgCl2 and 1.25–40 mM CaCl2) and high concentration monovalent cation (320–2560 mM NaCl) environments, which indicates that the added histidine residue conferred better salt-, acid- and alkali-tolerance to Pc-pis-His. Pc-pis-His also possessed the desired heat-tolerance, which was reflected by the antimicrobial activity of the peptide after being boiled for 10–60 minutes. Hemolytic activity analysis revealed that Pc-pis-His at concentrations up to 6 µM exhibited no hemolysis against human erythrocytes, with 6 µM being a concentration that is highly active against most of the microorganisms tested, although the hemolytic activity of Pc-pis-His was enhanced compared to Pc-pis. These results provide a unique, reasonable basis for designing novel piscidins with potent, broad-spectrum and stable antimicrobial activity and new insight into the future development of piscidins as potential therapeutic agents against microbial and external protozoan parasite infections.
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影响因子：
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作者：
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作者：
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作者：
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影响因子：
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作者：
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DOI：
10.1016/s0304-4157(98)00021-5
发表时间：
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期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON BIOMEMBRANES
影响因子：
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通讯作者：
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