The AS87_04050 gene is involved in bacterial lipopolysaccharide biosynthesis and pathogenicity of Riemerella anatipestifer.
The AS87_04050 gene is involved in bacterial lipopolysaccharide biosynthesis and pathogenicity of Riemerella anatipestifer.
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AS87_04050基因参与鸭疫里默氏菌的细菌脂多糖生物合成及致病性
DOI：
10.1371/journal.pone.0109962
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yu S
中科院分区：
文献类型：
--
作者：
Wang X;Ding C;Wang S;Han X;Hou W;Yue J;Zou J;Yu S
鸭疫里默氏菌是引起鸭败血症和渗出性疾病的主要病原。在本研究中，我们通过Tn4351转座子诱变获得了一株AS87_04050基因失活的突变株RA2640。Southern杂交分析表明，突变株RA2640的基因组中只有一个插入片段。突变菌株RA2640与野生型菌株Yb2的脂多糖(LPS)图谱不同，表明突变菌株RA2640的脂多糖存在缺陷。此外，突变菌株RA2640的菌落形态、自聚集能力和结晶紫染色特性也发生了明显的变化，表现为粗糙型。细菌内毒素是毒力的关键因素，也是宿主对感染的先天和后天反应的关键因素。粗糙型突变株RA2640对抗生素、消毒剂和正常鸭血清具有较高的敏感性，对Vero细胞的黏附和侵袭能力也高于野生型Yb2。此外，突变株RA2640失去了野生型菌株Yb2对2型鸭疫杆菌阳性血清的凝集能力，提示O-抗原存在缺陷。动物实验表明，与野生型菌株Yb2相比，突变菌株RA2640的毒力降低了10万倍以上。这些结果表明，鸭疫杆菌AS87_04050基因与内毒素的生物合成和细菌致病性有关。
Riemerella anatipestifer is reported worldwide as a cause of septicemic and exudative diseases of domestic ducks. In this study, we identified a mutant strain RA2640 by Tn4351 transposon mutagenesis, in which the AS87_04050 gene was inactivated by insertion of the transposon. Southern blot analysis indicated that only one insertion was found in the genome of the mutant strain RA2640. SDS-PAGE followed by silver staining showed that the lipopolysaccharide (LPS) pattern of mutant strain RA2640 was different from its wild-type strain Yb2, suggesting the LPS was defected. In addition, the phenotype of the mutant strain RA2640 was changed to rough-type, evident by altered colony morphology, autoaggregation ability and crystal violet staining characteristics. Bacterial LPS is a key factor in virulence as well as in both innate and acquired host responses to infection. The rough-type mutant strain RA2640 showed higher sensitivity to antibiotics, disinfectants and normal duck serum, and higher capability of adherence and invasion to Vero cells, compared to its wild-type strain Yb2. Moreover, the mutant strain RA2640 lost the agglutination ability of its wild-type strain Yb2 to R. anatipestifer serotype 2 positive sera, suggesting that the O-antigen is defected. Animal experiments indicated that the virulence of the mutant strain RA2640 was attenuated by more than 100,000-fold, compared to its wild-type strain Yb2. These results suggested that the AS87_04050 gene in R. anatipestifer is associated with the LPS biosynthesis and bacterial pathogenicity.
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通过随机转座子诱变鉴定参与鸭疫里默氏菌生物膜形成的基因
影响因子：
3.7
作者：
Hu Q;Zhu Y;Tu J;Yin Y;Wang X;Han X;Ding C;Zhang B;Yu S
通讯作者：
Yu S
影响因子：
3.2
作者：
Grozdanov, L;Zähringer, U;Dobrindt, U
通讯作者：
Dobrindt, U
影响因子：
3.6
作者：
DeShazer, D;Brett, PJ;Woods, DE
通讯作者：
Woods, DE
影响因子：
4.4
作者：
Alvarez, B.;Secades, P.;Guijarro, J. A.
通讯作者：
Guijarro, J. A.
影响因子：
3.1
作者：
MCSWEEGAN, E;WALKER, RI
通讯作者：
WALKER, RI