Membrane mechanical properties of synthetic asymmetric phospholipid vesicles.
Membrane mechanical properties of synthetic asymmetric phospholipid vesicles.
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DOI：
10.1039/c6sm01349j
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发表时间：
2016-09-13
期刊：
影响因子：
3.4
通讯作者：
Chiarot PR
中科院分区：
文献类型：
--
作者：
Lu L;Doak WJ;Schertzer JW;Chiarot PR
合成脂质囊泡已成为研究细胞膜生物学的重要模型系统。研究表明，双层膜的机械性能显著影响其生物学行为。脂双层的性质受脂酰基链长度、头基类型和膜蛋白的存在所控制。然而，很少有研究探讨膜结构，特别是跨双层脂质不对称性，影响膜的机械性能。在这项研究中，我们研究了脂质双层结构（即不对称性）对生物膜机械性能的影响。这是使用定制的微量移液管抽吸系统和我们的团队先前开发的用于构建具有定制双层结构的不对称磷脂囊泡的新型微流体技术实现的。我们发现，合成的非对称双层的弯曲模量和面积膨胀模量是高达50%以上的对称双层所获得的值。这是由不对称膜的双层的每个小叶中的不同脂质分布引起的。据我们所知，这是第一次报告的影响trans-bilayer不对称的合成双层膜的面积膨胀模量。由于双层膜的机械性能在许多细胞过程中起着重要作用，这些结果对膜生物学研究具有重要意义。
Synthetic lipid vesicles have served as important model systems to study cellular membrane biology. Research has shown that the mechanical properties of bilayer membranes significantly affects their biological behavior. The properties of a lipid bilayer are governed by lipid acyl chain length, headgroup type, and the presence of membrane proteins. However, few studies have explored how membrane architecture, in particular trans-bilayer lipid asymmetry, influences membrane mechanical properties. In this study, we investigated the effects of lipid bilayer architecture (i.e. asymmetry) on the mechanical properties of biological membranes. This was achieved using a customized micropipette aspiration system and a novel microfluidic technique previously developed by our team for building asymmetric phospholipid vesicles with tailored bilayer architecture. We found that the bending modulus and area expansion modulus of the synthetic asymmetric bilayers were up to 50% larger than the values acquired for symmetric bilayers. This was caused by the dissimilar lipid distribution in each leaflet of the bilayer for the asymmetric membrane. To the best of our knowledge, this is the first report on the impact of trans-bilayer asymmetry on the area expansion modulus of synthetic bilayer membranes. Since the mechanical properties of bilayer membranes play an important role in numerous cellular processes, these results have significant implications for membrane biology studies.
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