Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP). II. Role at Class I and class II CAP-dependent promoters.
Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP). II. Role at Class I and class II CAP-dependent promoters.
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大肠杆菌分解代谢物基因激活蛋白（CAP）激活区域的表征。
DOI：
10.1016/0022-2836(94)90035-3
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发表时间：
1994
影响因子：
5.6
通讯作者：
Ebright,RH
中科院分区：
文献类型：
--
作者：
Zhou,Y;Merkel,TJ;Ebright,RH
CAP依赖性启动子可以根据CAP的DNA位点的位置分为几类。在I类CAP依赖性启动子中，CAP的DNA位点位于RNA聚合酶的DNA位点的上游; CAP的DNA位点可以位于距转录起始点的各种距离处，条件是CAP的DNA位点和RNA聚合酶的DNA位点在DNA螺旋的同一面上。在II类CAP依赖性启动子中，CAP的DNA位点与RNA聚合酶的DNA位点重叠，取代了RNA聚合酶结合的−35个决定簇。在以前的工作中，我们已经表明，由氨基酸残基152至166 CAP的表面环是必不可少的转录激活在最好的表征I类CAP依赖性启动子，thelacpromoter，我们提出，这个表面环使直接蛋白质-蛋白质接触的三元复合体oflacpromoter，CAP和RNA聚合酶的RNA聚合酶。在这里，我们表明，由氨基酸残基152至166组成的表面环是必不可少的转录激活在其他I类CAP依赖性启动子和II类CAP依赖性启动子。我们进一步表明，残基152至166的丙氨酸取代的效果是定性相同的thelac启动子和其他I类CAP依赖性启动子，但在II类CAP依赖性启动子不同。我们建议，由残基152至166组成的表面环在所有I类CAP依赖性启动子的转录激活中产生相同的分子相互作用，无论CAP的DNA位点与转录起始点之间的距离如何，但在II类CAP依赖性启动子的转录激活中产生不同的分子相互作用。
CAP-dependent promoters can be divided into classes based on the position of the DNA site for CAP. In class I CAP-dependent promoters, the DNA site for CAP is located upstream of the DNA site for RNA polymerase; the DNA site for CAP can be located at various distances from the transcription start point, provided that the DNA site for CAP and the DNA site for RNA polymerase are on the same face of the DNA helix. In class II CAP-dependent promoters, the DNA site for CAP overlaps the DNA site for RNA polymerase, replacing the −35 determinants for binding of RNA polymerase. In previous work, we have shown that a surface loop consisting of amino acid residues 152 to 166 of CAP is essential for transcription activation at the best-characterized class I CAP-dependent promoter, thelacpromoter, and we proposed that this surface loop makes direct protein-protein contact with RNA polymerase in the ternary complex oflacpromoter, CAP, and RNA polymerase. Here, we show that the surface loop consisting of amino acid residues 152 to 166 is essential for transcription activation at other class I CAP-dependent promoters and at a class II CAP-dependent promoter. We show further that the effects of alanine substitutions of residues 152 to 166 are qualitatively identical at thelacpromoter and other class I CAP-dependent promoters, but are different at a class II CAP-dependent promoter. We propose that the surface loop consisting of residues 152 to 166 makes identical molecular interactions in transcription activation at all class I CAP-dependent promoters, irrespective of distance between the DNA site for CAP and the transcription start point, but makes a different set of molecular interactions in transcription activation at class II CAP-dependent promoters.
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大肠杆菌环 AMP 受体蛋白与 II 类启动子处的 RNA 聚合酶之间的相互作用
影响因子：
3.6
作者：
David West;Roy Williams;V. Rhodius;A. Bell;Naveen Sharma;C. Zou;N. Fujita;A. Ishihama;S. Busby
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
BUSBY, S
影响因子：
5.6
作者：
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通讯作者：
Wei Niu;Yuhong Zhou;Qianping Dong;Y. Ebright;R. Ebright
绘制大肠杆菌 RNA 聚合酶 α 亚基上的 cAMP 受体蛋白接触位点
影响因子：
3.6
作者：
C. Zou;N. Fujita;K. Igarashi;A. Ishihama
通讯作者：
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