Correlated retrograde and developmental regulons implicate multiple retrograde signals as coordinators of chloroplast development in maize
Correlated retrograde and developmental regulons implicate multiple retrograde signals as coordinators of chloroplast development in maize
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相关的逆行和发育调节子暗示多个逆行信号作为玉米叶绿体发育的协调者
DOI：
10.1093/plcell/koac276
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Barkan, Alice
中科院分区：
文献类型：
--
作者：
Kendrick, Rennie;Chotewutmontri, Prakitchai;Belcher, Susan;Barkan, Alice
叶绿体发出的信号影响核基因的表达，但逆行信号在叶绿体发育过程中的作用尚不清楚。为了解决这一差距，我们分析了非光合玉米突变体的转录组，并将其与正常叶片发育阶段的转录组进行了比较。两个缺乏质体核糖体的白化突变体的转录组与正常叶片发育早期的转录组相似，而两个具有类囊体靶向或质体转录缺陷的叶绿体突变体的转录组与稍晚阶段的转录组相似。我们确定了~ 2,700个差异表达基因，根据极性和突变特异性的变化分为六大类。下调基因通常在正常发育后期表达，并在光合作用基因中富集，而上调基因则在早期作用，并在叶绿体生物发生和细胞质翻译中富集。我们进一步发现，雷帕霉素靶（TOR）信号在缺乏质体核糖体的突变体中升高，并在正常叶片发育过程中随着质体核糖体的积累而下降。我们的结果暗示三个质体信号作为光合分化的协调者。一个信号需要质体核糖体并激活光合作用基因。第二个信号反映了叶绿体成熟的实现和抑制叶绿体生物发生基因。第三种信号是叶绿体发育过程中的营养消耗，抑制TOR，促进叶片发育过程中细胞增殖的终止。
Signals emanating from chloroplasts influence nuclear gene expression, but roles of retrograde signals during chloroplast development are unclear. To address this gap, we analyzed transcriptomes of non-photosynthetic maize mutants and compared them to transcriptomes of stages of normal leaf development. The transcriptomes of two albino mutants lacking plastid ribosomes resembled transcriptomes at very early stages of normal leaf development, whereas the transcriptomes of two chlorotic mutants with thylakoid targeting or plastid transcription defects resembled those at a slightly later stage. We identified ∼2,700 differentially expressed genes, which fall into six major categories based on the polarity and mutant-specificity of the change. Downregulated genes were generally expressed late in normal development and were enriched in photosynthesis genes, whereas upregulated genes act early and were enriched for functions in chloroplast biogenesis and cytosolic translation. We showed further that target-of-rapamycin (TOR) signaling was elevated in mutants lacking plastid ribosomes and declined in concert with plastid ribosome buildup during normal leaf development. Our results implicate three plastid signals as coordinators of photosynthetic differentiation. One signal requires plastid ribosomes and activates photosynthesis genes. A second signal reflects attainment of chloroplast maturity and represses chloroplast biogenesis genes. A third signal, the consumption of nutrients by developing chloroplasts, represses TOR, promoting termination of cell proliferation during leaf development.
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影响因子：
14.9
作者：
Rojas M;Ruwe H;Miranda RG;Zoschke R;Hase N;Schmitz-Linneweber C;Barkan A
通讯作者：
Barkan A
影响因子：
7.4
作者：
R. Williams-Carrier;Reimo Zoschke;Susan Belcher;J. Pfalz;A. Barkan
通讯作者：
R. Williams-Carrier;Reimo Zoschke;Susan Belcher;J. Pfalz;A. Barkan
DOI：
10.1111/tpj.12011
发表时间：
2013-01
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Woodson JD;Perez-Ruiz JM;Schmitz RJ;Ecker JR;Chory J
通讯作者：
Chory J
影响因子：
6.4
作者：
Brunkard JO;Burch-Smith TM
通讯作者：
Burch-Smith TM
GUN1 调节拟南芥中的四吡咯生物合成。
DOI：
--
发表时间：
2019
期刊：
影响因子：
--
作者：
Masuda T;Shimizu T;Mochizuki N;Nagatani A;Watanabe S;Kacprzak S;Okamoto H;Terry MJ
通讯作者：
Terry MJ