Unexpected functional versatility of the pentatricopeptide repeat proteins PGR3, PPR5 and PPR10.
Unexpected functional versatility of the pentatricopeptide repeat proteins PGR3, PPR5 and PPR10.
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DOI：
10.1093/nar/gky737
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发表时间：
2018-11-02
影响因子：
14.9
通讯作者：
Barkan A
中科院分区：
文献类型：
--
作者：
Rojas M;Ruwe H;Miranda RG;Zoschke R;Hase N;Schmitz-Linneweber C;Barkan A
五肽重复(PPR)蛋白是一个螺旋重复蛋白大家族，与线粒体和叶绿体中的RNA结合。PPR的作用部位主要是从遗传数据中推断出来的，这导致了这样一种观点，即大多数PPR蛋白在体内只作用于极少数的部位。在这里，我们报告了三个已被深入研究的叶绿体PPR蛋白的新功能。之前显示促进trnG剪接的玉米PPR5也是rpl16剪接所必需的。玉米PPR10结合了ATPI-atpH和psaJ-rpl33顺反子间区，也稳定了psaI下游的3‘端。拟南芥PGR3以前被证明结合在Pet1的上游，也结合了rpl14-rps8顺反子间区，在那里它稳定了3‘端并刺激了rps8的翻译。PGR3的这些功能在玉米中是保守的。已经是PPR家族中特征最好的成员之一的三种蛋白质的新功能的发现表明，PPR蛋白质的功能谱比人们所了解的要复杂得多。体内PPR10和PGR3结合序列的多样性突显了基于PPR基序识别核苷酸的氨基酸编码预测天然PPR蛋白结合位点的挑战。
Pentatricopeptide repeat (PPR) proteins are a large family of helical repeat proteins that bind RNA in mitochondria and chloroplasts. Sites of PPR action have been inferred primarily from genetic data, which have led to the view that most PPR proteins act at a very small number of sites in vivo. Here, we report new functions for three chloroplast PPR proteins that had already been studied in depth. Maize PPR5, previously shown to promote trnG splicing, is also required for rpl16 splicing. Maize PPR10, previously shown to bind the atpI-atpH and psaJ-rpl33 intercistronic regions, also stabilizes a 3′-end downstream from psaI. Arabidopsis PGR3, shown previously to bind upstream of petL, also binds the rpl14-rps8 intercistronic region where it stabilizes a 3′-end and stimulates rps8 translation. These functions of PGR3 are conserved in maize. The discovery of new functions for three proteins that were already among the best characterized members of the PPR family implies that functional repertoires of PPR proteins are more complex than have been appreciated. The diversity of sequences bound by PPR10 and PGR3 in vivo highlights challenges of predicting binding sites of native PPR proteins based on the amino acid code for nucleotide recognition by PPR motifs.
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影响因子：
11.6
作者：
McCormac, DJ;Barkan, A
通讯作者：
Barkan, A
DOI：
10.1261/rna.059568.116
发表时间：
2017-04
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Miranda RG;Rojas M;Montgomery MP;Gribbin KP;Barkan A
通讯作者：
Barkan A
影响因子：
11.6
作者：
Douchi, Damien;Qu, Yujiao;Goldschmidt-Clermont, Michel
通讯作者：
Goldschmidt-Clermont, Michel
影响因子：
4.5
作者：
Chotewutmontri P;Barkan A
通讯作者：
Barkan A
影响因子：
7.4
作者：
Liu, Xiayan;Yu, Fei;Rodermel, Steve
通讯作者：
Rodermel, Steve