Spatial and temporal distribution of SARS-CoV-2 diversity circulating in wastewater.

Spatial and temporal distribution of SARS-CoV-2 diversity circulating in wastewater.
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DOI:
10.1016/j.watres.2021.118007
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发表时间:
2022-03-01
期刊:
影响因子:
12.8
通讯作者:
Sánchez G
Sánchez G
中科院分区:
环境科学与生态学1区
文献类型:
--
作者:
Pérez-Cataluña A;Chiner-Oms Á;Cuevas-Ferrando E;Díaz-Reolid A;Falcó I;Randazzo W;Girón-Guzmán I;Allende A;Bracho MA;Comas I;Sánchez G

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在当前的COVID-19大流行期间,基于水的流行病学(WBE)已被证明是SARS-CoV-2流行病学监测的有效工具。此外,将WBE与高通量测序技术相结合,可用于分析给定样品中存在的SARS-CoV-2病毒多样性。本研究的重点是SARS-CoV-2的基因组分析在西班牙发生的三个流行病学浪潮期间收集的76个污水样本分布在全国各地的14个污水处理厂。所获得的结果表明,废水中SARS-CoV-2的宏基因组分析允许检测定义B.1.1.7谱系的突变,并且该技术能够在临床样本中检测到某些突变之前预测它们的检测。该研究证明了污水测序在跟踪关注变体方面的有用性,这些变体可以补充临床测试,以帮助决策和分析大流行的演变。
Wastewater-based epidemiology (WBE) has proven to be an effective tool for epidemiological surveillance of SARS-CoV-2 during the current COVID-19 pandemic. Furthermore, combining WBE together with high-throughput sequencing techniques can be useful for the analysis of SARS-CoV-2 viral diversity present in a given sample. The present study focuses on the genomic analysis of SARS-CoV-2 in 76 sewage samples collected during the three epidemiological waves that occurred in Spain from 14 wastewater treatment plants distributed throughout the country. The results obtained demonstrate that the metagenomic analysis of SARS-CoV-2 in wastewater allows the detection of mutations that define the B.1.1.7 lineage and the ability of the technique to anticipate the detection of certain mutations before they are detected in clinical samples. The study proves the usefulness of sewage sequencing to track Variants of Concern that can complement clinical testing to help in decision-making and in the analysis of the evolution of the pandemic.
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