Induction of PtoCDKB and PtoCYCB transcription by temperature during cambium reactivation in Populus tomentosa Carr.
Induction of PtoCDKB and PtoCYCB transcription by temperature during cambium reactivation in Populus tomentosa Carr.
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毛白杨卡尔形成层再激活过程中温度对 PtoCDKB 和 PtoCYCB 转录的诱导。
DOI：
10.1093/jxb/erp108
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发表时间：
2009
影响因子：
6.9
通讯作者：
He XQ
中科院分区：
文献类型：
--
作者：
Li WF;Ding Q;Chen JJ;Cui KM;He XQ
细胞周期进程需要细胞周期蛋白依赖性激酶B（CDKB）和细胞周期蛋白B（CYCB）之间的相互作用。毛白杨（Populus tomentosa）卡尔（Carr.）在水培条件下，进一步研究了温度和外源吲哚乙酸（IAA）对其表达的影响。根据休眠形成层细胞对外源IAA的不同反应，将毛白杨形成层休眠分为4个阶段：静止1期（Q1）、休眠期、静止2-1期（Q2-1）和静止2-2期（Q2-2）。PtoCDKB和PtoCYCB转录本在活动期强烈表达，在Q1中弱表达，并且从休息直到Q2-2晚期几乎检测不到。气候数据分析表明，每日气温和PtoCDKB和PtoCYCB表达模式之间的相关性。温度处理的水培养实验进一步表明，低温（4 °C）使PtoCDKB和PtoCYCB转录物保持在不可检测的水平，而温暖的温度（25 °C）诱导它们在形成层区域中的表达。同时，在水培条件下，外源IAA对PtoCDKB和PtoCYCB基因转录的诱导不依赖于外源IAA。结果表明，早春温度是温带落叶阔叶毛白杨形成层复活的重要环境因子。早春温度升高可能诱导形成层区域的CDKB和CYCB同源物转录，这是形成层细胞分裂所必需的。
Cell cycle progression requires interaction between cyclin-dependent kinase B (CDKB) and cyclin B (CYCB). The seasonal expression patterns of the CDKB and CYCB homologues from Populus tomentosa Carr. were investigated, and effects of temperature and exogenous indole-3-acetic acid (IAA) on their expression were further studied in water culture experiments. Based on the differential responses of dormant cambium cells to exogenous IAA, four stages of cambium dormancy were confirmed for P. tomentosa: quiescence 1 (Q1), rest, quiescence 2-1 (Q2-1), and quiescence 2-2 (Q2-2). PtoCDKB and PtoCYCB transcripts were strongly expressed in the active phases, weakly in Q1, and almost undetectable from rest until late Q2-2. Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns. Water culture experiments with temperature treatment further showed that a low temperature (4 °C) kept PtoCDKB and PtoCYCB transcripts at undetectable levels, while a warm temperature (25 °C) induced their expression in the cambium region. Meanwhile, water culture experiments with exogenous IAA treatment showed that induction of PtoCDKB and PtoCYCB transcription was independent of exogenous IAA. The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation. The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.
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影响因子：
4.2
作者：
Gricar, Jozica;Zupancic, Martin;Oven, Primoz
通讯作者：
Oven, Primoz
影响因子：
4.3
作者：
Oribe, Y;Funada, R;Kubo, T
通讯作者：
Kubo, T
影响因子：
2.3
作者：
Oribe, Y;Funada, R;Kubo, T
通讯作者：
Kubo, T
影响因子：
11.6
作者：
HIRT, H;MINK, M;HEBERLEBORS, E
通讯作者：
HEBERLEBORS, E
杜仲中 IAA 和 ABA 调节的 ABP1 表达与形成层周期性相关。
影响因子：
6.9
作者：
Hou, Hong-Wei;Zhou, Yu-Ting;Cui, Ke-Ming
通讯作者：
Cui, Ke-Ming