Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit.
Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit.
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DOI：
10.1016/j.jviromet.2020.114024
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发表时间：
2021-03
影响因子：
3.1
通讯作者：
Kleines M
中科院分区：
文献类型：
--
作者：
Krüttgen A;Cornelissen CG;Dreher M;Hornef MW;Imöhl M;Kleines M
评估了新型罗氏SARS-CoV-2快速抗原检测的灵敏度和特异性。特异性为96%。Ct = 35的样品的灵敏度< 25 was 100 % and gradually decreases to 22.2 % with cycle thresholds >。新的测试可能有助于快速识别传染性个体。目前需要可靠的抗原检测方法，以及时和方便地检测急性SARS-CoV-2感染的个体。使用75份来自SARS-CoV-2 PCR检测阳性患者的拭子和75份来自SARS-CoV-2 PCR检测阴性患者的拭子，我们研究了SARS-CoV-2快速抗原检测（Roche）的灵敏度和特异性。我们确定特异性为96%。对于循环阈值< 25、25 - <30、30 - <35和> = 35的样品，测定的灵敏度分别为100%、95%、44.8%和22.2%。我们的结论是，抗原检测的敏感性和特异性是劣于PCR检测。然而，抗原检测可能是区分SARS-CoV-2传染性个体与非传染性个体的一种快速简便的替代方法。
The sensitivity and specificity of the new Roche SARS-CoV-2 Rapid Antigen Test was evaluated. We found a specificity of 96 %. The sensitivity with samples with a Ct of < 25 was 100 % and gradually decreases to 22.2 % with cycle thresholds > = 35. The new test might be useful to rapidly identify contagious individuals. There is an ongoing need for reliable antigen assays for timely and easy detection of individuals with acute SARS-CoV-2 infection. Using 75 swabs from patients previously tested positive by SARS-CoV-2 PCR and 75 swabs from patients previously tested negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the SARS-CoV-2 Rapid Antigen Test (Roche). We determined a specificity of 96 %. The assay’s sensitivity with samples with a cycle threshold of < 25, 25 - <30, 30 - <35, and> = 35 was 100 %, 95 %, 44.8 % and 22.2 %, respectively. We conclude that sensitivity and specificity of the antigen assay is inferior to the PCR assay. However, the antigen assay may be a quick and easy to perform alternative for differentiation of individuals contagious for SARS-CoV-2 from non-contagious individuals.
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影响因子：
8.8
作者：
Eis-Huebinger, Anna M.;Hoenemann, Mario;Panning, Marcus
通讯作者：
Panning, Marcus
影响因子：
3.1
作者：
Krüttgen A;Cornelissen CG;Dreher M;Hornef MW;Imöhl M;Kleines M
通讯作者：
Kleines M
DOI：
10.1016/j.jcv.2020.104412
发表时间：
2020-07-01
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
van Kasteren, Puck B;van der Veer, Bas;Meijer, Adam
通讯作者：
Meijer, Adam
DOI：
10.1016/j.jcv.2020.104654
发表时间：
2020-11
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Cerutti F;Burdino E;Milia MG;Allice T;Gregori G;Bruzzone B;Ghisetti V
通讯作者：
Ghisetti V
影响因子：
158.5
作者：
Zhu, Na;Zhang, Dingyu;Tan, Wenjie
通讯作者：
Tan, Wenjie