Decontamination treatments to eliminate problem biota from macroalgal tank cultures of Osmundea pinnatifida, Palmaria palmata and Ulva lactuca.

Decontamination treatments to eliminate problem biota from macroalgal tank cultures of Osmundea pinnatifida, Palmaria palmata and Ulva lactuca.
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从Osmundea Pinnatifida,Palmaria Palmata和Ulva lactuca的大藻罐培养物中消除问题生物群的去污染处理。

DOI:
10.1007/s10811-016-0873-9
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发表时间:
2016
影响因子:
3.3
通讯作者:
Hughes, Adam D.
Hughes, Adam D.
中科院分区:
生物学3区
文献类型:
--
作者:
Kerrison, Philip D.;Le, Hau Nhu;Twigg, Gail C.;Smallman, Duncan R.;MacPhee, Rory;Houston, Fiona A. B.;Hughes, Adam D.

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研究了不同浓度和暴露时间的化学消毒剂对5种大型海藻和海洋腹足类滨螺的消毒效果。Palmaria palmata、Osmundea pinnatifida和Ulva lactuca具有商业价值,并且通常在用于食物或饲料的罐中栽培。石莼(Ulva palmata)和石莼(O. siliculosus)是掌叶石莼(P. palmata)和石莼(O. palmata)的常见附生植物。pinnatifida文化,而Littorina spp.是美国常见的食草性附生生物。莴苣培养罐。这些污染物降低了作为食物的培养物的生产力和质量。使用脉冲幅度调制(PAM)叶绿素a荧光,几个小时和一个星期后处理的藻类物种之间的差异耐受性的治疗。我们确定的治疗,选择性地破坏了真菌,但不是底生植物物种。硅果香豆对1%次氯酸钠的耐受性显著低于掌叶香豆,对25%甲醇的耐受性显著低于掌叶香豆。pinnatifida,暴露1-5分钟似乎是最合适的。石莼的耐受性显著低于掌叶石莼和石莼; pinnatifida对许多消毒剂:0.1- 1%次氯酸钠10分钟,0.5%碘化钾最多10分钟,和0.25% Kick-start(商业水产养殖消毒剂溶液)1-5分钟。没有一种治疗方法能够杀死腹足类蜗牛而不损害美国的健康。虽然在淡水中搅动一小时可能会使它们从底生植物上分离,但对美国大豆几乎没有生理影响。莴苣该实验为更广泛的商业试验奠定了基础。
The effect of a range of chemical disinfectants at different concentration and exposure times was investigated on five macroalgal species and the marine gastropod Littorina spp. Palmaria palmata, Osmundea pinnatifida and Ulva lactuca are commercially valuable and are often cultivated in tanks for food or feed. Ectocarpus siliculosus and Ulva intestinalis are common epiphytes of P. palmata and O. pinnatifida cultures, whilst Littorina spp. are common herbivorous epibionts within U. lactuca culture tanks. These contaminants reduce the productivity and quality of the culture as a food. Differential tolerance to the treatments was seen between the algal species using pulse-amplitude modulation (PAM) chlorophyll a fluorescence, a few hours and a week following treatment. We identified treatments that selectively damaged the epiphyte but not the basiphyte species. Ectocarpus siliculosus had a significantly lower tolerance to 1 % sodium hypochlorite than P. palmata, and to 25 % methanol than O. pinnatifida, with a 1–5 min exposure appearing most suitable. Ulva intestinalis had a significantly lower tolerance than P. palmata and O. pinnatifida to many disinfectants: 0.1–1 % sodium hypochlorite for 10 min, 0.5 % potassium iodide for up to 10 min, and 0.25 % Kick-start (a commercial aquaculture disinfectant solution) for 1–5 min. No treatment was able to kill the gastropod snails without also damaging U. lactuca, although agitation in freshwater for an hr may cause them to detach from the basiphyte, with little to no photophysiological impact seen to U. lactuca. This experiment forms the basis for more extended commercial trials.
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